ITGB3BP sirna

ITGB3BP siRNA (Rat)

Cat. Num. AAA8219395

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ITGB3BP; ITGB3BP siRNA (Rat); CENPR; NRIF3; Centromere protein R; CENP-R; Nuclear receptor-interacting factor 3; ITGB3BP sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ITGB3BP siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 176

• Applicable Applications for ITGB3BP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ITGB3BP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ITGB3BP sirna

siRNA to inhibit ITGB3BP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61557307
• NCBI GeneID: 362548
• NCBI Accession #: NP_001013231.1
• NCBI GenBank Nucleotide #: NM_001013213.1
• UniProt Accession #: Q5U1Z7
• Molecular Weight: 19,917 Da
• NCBI Official Full Name: centromere protein R
• NCBI Official Synonym Full Names: integrin beta 3 binding protein (beta3-endonexin)
• NCBI Official Symbol: Itgb3bp
• NCBI Protein Information: centromere protein R
• UniProt Protein Name: Centromere protein R
• Protein Family: Centromere protein
• UniProt Gene Name: Itgb3bp
• UniProt Synonym Gene Names: Cenpr; Nrif3; CENP-R
• UniProt Entry Name: CENPR_RAT

Uniprot Description

NRIF3: Transcription coregulator that can have both coactivator and corepressor functions. Isoform 1, but not other isoforms, is involved in the coactivation of nuclear receptors for retinoid X (RXRs) and thyroid hormone (TRs) in a ligand-dependent fashion. In contrast, it does not coactivate nuclear receptors for retinoic acid, vitamin D, progesterone receptor, nor glucocorticoid. Acts as a coactivator for estrogen receptor alpha. Acts as a transcriptional corepressor via its interaction with the NFKB1 NF- kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. Induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. May also act as an inhibitor of cyclin A- associated kinase. Also acts a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Homodimer; mediated by the coiled coil domain. Isoform 3, but not other isoforms, interacts with the cytoplasmic tail of integrin ITGB3. The relevance of the interaction with ITGB3 is however uncertain, since isoform 3 is mainly nuclear. Interacts with CCNA2 and MTA1. Interacts with NFKB1 NF-kappa-B subunit. Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. By estrogen. Widely expressed. Expressed in spleen, thymus, prostate, ovary, small intestine and white blood cells. Highly expressed in testis and colon. Isoform 4 is expressed in platelets, lymphocytes and granulocytes. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Cell adhesion; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Cellular Component: nucleoplasm; nucleus

Biological Process: mitosis; DNA replication-independent nucleosome assembly at centromere; transcription, DNA-dependent; regulation of transcription, DNA-dependent; cell division; apoptosis