GIT1 sirna

GIT1 siRNA (Rat)

Cat. Num. AAA8218819

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: GIT1; GIT1 siRNA (Rat); ARF GTPase-activating protein GIT1; ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1; GIT1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: GIT1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 770

• Applicable Applications for GIT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat GIT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Ships on blue ice. Store at -20 degree C for one year.

Related Product Information for GIT1 sirna

siRNA to inhibit GIT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13929158
• NCBI GeneID: 83709
• NCBI Accession #: NP_114002.1
• NCBI GenBank Nucleotide #: NM_031814.1
• UniProt Accession #: Q9Z272
• NCBI Official Full Name: ARF GTPase-activating protein GIT1
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase interacting ArfGAP 1
• NCBI Official Symbol: Git1
• NCBI Protein Information: ARF GTPase-activating protein GIT1
• UniProt Protein Name: ARF GTPase-activating protein GIT1
• Protein Family: ARF GTPase-activating protein
• UniProt Gene Name: Git1
• UniProt Synonym Gene Names: ARF GAP GIT1; CAT-1; CAT1
• UniProt Entry Name: GIT1_RAT

NCBI Description

mediates beta2-adrenergic receptor endocytosis [RGD, Feb 2006]

Uniprot Description

GIT1: a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs).

Protein type: GAPs; Motility/polarity/chemotaxis; GAPs, ARF

Cellular Component: focal adhesion; membrane; cytoplasm

Molecular Function: small GTPase binding; protein binding; protein homodimerization activity; zinc ion binding; protein complex binding; GTPase activator activity

Biological Process: formation of immunological synapse; ephrin receptor signaling pathway; regulation of cytokinesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity