UCK1 sirna

UCK1 siRNA (Human)

Cat. Num. AAA8218766

• Gene Names: UCK1; URK1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UCK1; UCK1 siRNA (Human); URK1; Uridine-cytidine kinase 1; UCK 1; Cytidine monophosphokinase 1; Uridine monophosphokinase 1; UCK1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: UCK1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 201

• Applicable Applications for UCK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human UCK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UCK1 sirna

siRNA to inhibit UCK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 209863022
• NCBI GeneID: 83549
• NCBI Accession #: NP_001129426.1
• NCBI GenBank Nucleotide #: NM_001135954.1
• UniProt Accession #: Q9HA47; Q5JT09; Q5JT10; Q5JT12; Q5JT13; Q6IA74; Q96BJ0
• Molecular Weight: 32,275 Da
• NCBI Official Full Name: uridine-cytidine kinase 1 isoform b
• NCBI Official Synonym Full Names: uridine-cytidine kinase 1
• NCBI Official Symbol: UCK1
• NCBI Official Synonym Symbols: URK1
• NCBI Protein Information: uridine-cytidine kinase 1
• UniProt Protein Name: Uridine-cytidine kinase 1
• Protein Family: Uridine-cytidine kinase
• UniProt Gene Name: UCK1
• UniProt Synonym Gene Names: URK1; UCK 1
• UniProt Entry Name: UCK1_HUMAN

NCBI Description

This gene encodes a uridine-cytidine kinase that catalyzes the phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP) but not the phosphorylation of deoxyribonucleosides or purine ribonucleosides. This enzyme can also phosphorylate uridine and cytidine analogs and uses both ATP and GTP as a phosphate donor. Alternative splicing results in multiple splice variants encoding distinct isoforms. [provided by RefSeq, May 2012]

Uniprot Description

UCK1: Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4- thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)- benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5- methylcytidine, and N(4)-anisoylcytidine. Belongs to the uridine kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.48; Kinase, other; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: cytosol

Molecular Function: nucleoside kinase activity; uridine kinase activity; ATP binding

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; phosphorylation