TDG sirna

TDG siRNA (Human)

Cat. Num. AAA8218567

• Gene Names: TDG; hTDG
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TDG; TDG siRNA (Human); G/T mismatch-specific thymine DNA glycosylase; Thymine-DNA glycosylase; hTDG; TDG sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TDG siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 410

• Applicable Applications for TDG sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TDG gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TDG sirna

siRNA to inhibit TDG expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 59853162
• NCBI GeneID: 6996
• NCBI Accession #: NP_003202.3
• NCBI GenBank Nucleotide #: NM_003211.4
• UniProt Accession #: Q13569; Q8IUZ6; Q8IZM3
• Molecular Weight: 46,053 Da
• NCBI Official Full Name: G/T mismatch-specific thymine DNA glycosylase
• NCBI Official Synonym Full Names: thymine DNA glycosylase
• NCBI Official Symbol: TDG
• NCBI Official Synonym Symbols: hTDG
• NCBI Protein Information: G/T mismatch-specific thymine DNA glycosylase
• UniProt Protein Name: G/T mismatch-specific thymine DNA glycosylase
• Protein Family: G/T mismatch-specific thymine DNA glycosylase
• UniProt Gene Name: TDG
• UniProt Synonym Gene Names: hTDG
• UniProt Entry Name: TDG_HUMAN

NCBI Description

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]

Uniprot Description

TDG: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. Belongs to the TDG/mug DNA glycosylase family.

Protein type: Hydrolase; EC 3.2.2.29; Nuclear receptor co-regulator; DNA repair, damage

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; PML body; plasma membrane; nucleus

Molecular Function: protein domain specific binding; protein binding; structure-specific DNA binding; protein homodimerization activity; SUMO binding; protein kinase C binding; DNA N-glycosylase activity; double-stranded DNA binding; damaged DNA binding; transcription factor binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; mismatched DNA binding

Biological Process: transcription from RNA polymerase II promoter; embryonic development; mismatch repair; base-excision repair, AP site formation; depyrimidination; base-excision repair; gene expression; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; DNA repair; negative regulation of protein binding; regulation of gene expression, epigenetic