RNF2 sirna

RNF2 siRNA (Mouse)

Cat. Num. AAA8217652

• Gene Names: Rnf2; dinG; Ring1B; AI326319; AI450156; AU019207
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RNF2; RNF2 siRNA (Mouse); DING; RING1B; E3 ubiquitin-protein ligase RING2; RING finger protein 1B; RING1b; RING finger protein 2; RNF2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RNF2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 336

• Applicable Applications for RNF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RNF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RNF2 sirna

siRNA to inhibit RNF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 33563274
• NCBI GeneID: 19821
• NCBI Accession #: NP_035407.1
• NCBI GenBank Nucleotide #: NM_011277.2
• UniProt Accession #: Q9CQJ4; O35699; O35729; Q4FJV5; Q8C1X8
• Molecular Weight: 37,623 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RING2
• NCBI Official Synonym Full Names: ring finger protein 2
• NCBI Official Symbol: Rnf2
• NCBI Official Synonym Symbols: dinG; Ring1B; AI326319; AI450156; AU019207
• NCBI Protein Information: E3 ubiquitin-protein ligase RING2
• UniProt Protein Name: E3 ubiquitin-protein ligase RING2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Rnf2
• UniProt Synonym Gene Names: DinG; Ring1b; RING1b
• UniProt Entry Name: RING2_MOUSE

Uniprot Description

RNF2: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin- protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. Component of chromatin-associated Polycomb (PcG) complexes. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2. Component of a PRC1-like complex. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with RYBP, HIP2 and TFCP2. Association to the chromosomal DNA is cell-cycle dependent. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1. Interacts with Interacts with PCGF2, CBX4, CBX6, CBX7 and CBX8. Interacts with CBX2, BMI and PHC2. Interacts with RYBP, HIP2 and TFCP2.

Protein type: EC 6.3.2.-; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Cellular Component: nucleoplasm; nuclear body; heterochromatin; euchromatin; sex chromatin; chromosome; PcG protein complex; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; chromatin binding; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; histone ubiquitination; mitotic cell cycle; gastrulation with mouth forming second; negative regulation of transcription from RNA polymerase II promoter; anterior/posterior axis specification