ATP2B2 sirna

ATP2B2 siRNA (Rat)

Cat. Num. AAA8217546

• Gene Names: Atp2b2; PMCA2
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP2B2; ATP2B2 siRNA (Rat); Plasma membrane calcium-transporting ATPase 2; PMCA2; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2; ATP2B2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ATP2B2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,198

• Applicable Applications for ATP2B2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ATP2B2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP2B2 sirna

siRNA to inhibit ATP2B2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978557
• NCBI GeneID: 24215
• NCBI Accession #: NP_036640.1
• NCBI GenBank Nucleotide #: NM_012508.5
• UniProt Accession #: P11506; Q63443
• Molecular Weight: 132,616 Da
• NCBI Official Full Name: plasma membrane calcium-transporting ATPase 2
• NCBI Official Synonym Full Names: ATPase, Ca++ transporting, plasma membrane 2
• NCBI Official Symbol: Atp2b2
• NCBI Official Synonym Symbols: PMCA2
• NCBI Protein Information: plasma membrane calcium-transporting ATPase 2
• UniProt Protein Name: Plasma membrane calcium-transporting ATPase 2
• Protein Family: Plasma membrane calcium-transporting ATPase
• UniProt Gene Name: Atp2b2
• UniProt Synonym Gene Names: PMCA2
• UniProt Entry Name: AT2B2_RAT

NCBI Description

regulates intracellular calcium ion levels [RGD, Feb 2006]

Uniprot Description

ATP2B2: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.6.3.8; Cell development/differentiation; Transporter, ion channel; Transporter; Membrane protein, integral; Endoplasmic reticulum; Membrane protein, multi-pass

Cellular Component: cell; endoplasmic reticulum; dendrite; integral to membrane; cytosol; lipid raft; cilium; cell soma; apical plasma membrane; cytoplasm; plasma membrane; synapse; cell junction

Molecular Function: protein C-terminus binding; calmodulin binding; glutamate receptor binding; calcium-transporting ATPase activity; metal ion binding; acetylcholine receptor binding; calcium ion binding; ATP binding; calcium-dependent ATPase activity; PDZ domain binding

Biological Process: lactation; metabolic process; cell morphogenesis; regulation of synaptic plasticity; locomotory behavior; neuron differentiation; sensory perception of sound; inner ear receptor cell differentiation; calcium ion transport; synapse organization and biogenesis; cerebellum development; auditory receptor cell stereocilium organization and biogenesis; locomotion; neuromuscular process controlling balance; inner ear development; cGMP metabolic process; serotonin metabolic process; inner ear morphogenesis; cytosolic calcium ion homeostasis; otolith mineralization; organelle organization and biogenesis; detection of mechanical stimulus involved in sensory perception of sound; cerebellar granule cell differentiation; cellular calcium ion homeostasis; cerebellar Purkinje cell differentiation; brain development; positive regulation of calcium ion transport; cerebellar Purkinje cell layer morphogenesis; regulation of cell size