JARID2 sirna

JARID2 siRNA (Mouse)

Cat. Num. AAA8217270

• Gene Names: Jarid2; Jmj; jumonji
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: JARID2; JARID2 siRNA (Mouse); JMJ; Protein Jumonji; Jumonji/ARID domain-containing protein 2; JARID2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: JARID2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,196

• Applicable Applications for JARID2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse JARID2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for JARID2 sirna

siRNA to inhibit JARID2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 326537277
• NCBI GeneID: 16468
• NCBI Accession #: NP_001191972.1
• NCBI GenBank Nucleotide #: NM_001205043.1
• UniProt Accession #: Q62315; Q3TPU4; Q3UHS7; Q99LD1
• Molecular Weight: 132,938 Da
• NCBI Official Full Name: protein Jumonji
• NCBI Official Synonym Full Names: jumonji, AT rich interactive domain 2
• NCBI Official Symbol: Jarid2
• NCBI Official Synonym Symbols: Jmj; jumonji
• NCBI Protein Information: protein Jumonji
• UniProt Protein Name: Protein Jumonji
• UniProt Gene Name: Jarid2
• UniProt Synonym Gene Names: Jmj
• UniProt Entry Name: JARD2_MOUSE

Uniprot Description

JARID2: a Polycomb protein required for neural tube formation. Contains a Jumonji C domain but lacks histone demethylase activity. Essential for normal heart development and function. Required for embryonic viability. Down-regulation of Jarid2 impairs ES cell differentiation and Oct4 silencing. Associates with the Polycomb-Repressive Complex 2 (PRC2), stimulating PRC2 methyltransferase activity. PRC2 includes the histone H3 Lys 27 (H3K27) methyltransferase Ezh2, EED, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The recruitment of PRC2 to a promoter leads to increased levels of di- and trimethylation of H3K27 (H3K27me2/3), regulating the balance between self-renewal and differentiation of embryonic stem cells. The ability of Jarid 2 to be recruited to DNA in ES cells is interdependent on PRC2. Its knockdown reduces PRC2 at its target promoters, while ES cells lacking the PRC2 component EED are deficient in Jarid2 promoter activity. Acts as a transcriptional repressor of ANF by binding to both GATA4 and NKX2-5 and repressing their transcriptional activator activities. Participates in the negative regulation of cell proliferation signaling. Interacts with the N-terminal region of GATA4 and the C- terminal region of the NKX2-5 homeobox and represses their ability to activate transcription of ANF.

Protein type: Transcription, coactivator/corepressor

Cellular Component: histone methyltransferase complex; ESC/E(Z) complex; nucleus

Molecular Function: protein binding; DNA binding; histone demethylase activity; chromatin binding

Biological Process: positive regulation of histone H3-K9 methylation; transcription from RNA polymerase II promoter; spleen development; transcription, DNA-dependent; thymus development; multicellular organismal development; stem cell differentiation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; liver development; negative regulation of histone methylation; regulation of cell proliferation; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; cell differentiation; negative regulation of transcription, DNA-dependent