RSPH4A sirna

RSPH4A siRNA (Mouse)

Cat. Num. AAA8217239

• Gene Names: Rsph4a; Rshl3; A230081C05
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RSPH4A; RSPH4A siRNA (Mouse); RSHL3; Radial spoke head protein 4 homolog A; Radial spoke head-like protein 3; RSPH4A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RSPH4A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 716

• Applicable Applications for RSPH4A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RSPH4A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RSPH4A sirna

siRNA to inhibit RSPH4A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 242397427
• NCBI GeneID: 212892
• NCBI Accession #: NP_001156429.1
• NCBI GenBank Nucleotide #: NM_001162957.1
• UniProt Accession #: Q8BYM7; B2RQN0
• Molecular Weight: 80,146 Da
• NCBI Official Full Name: radial spoke head protein 4 homolog A
• NCBI Official Synonym Full Names: radial spoke head 4 homolog A (Chlamydomonas)
• NCBI Official Symbol: Rsph4a
• NCBI Official Synonym Symbols: Rshl3; A230081C05
• NCBI Protein Information: radial spoke head protein 4 homolog A
• UniProt Protein Name: Radial spoke head protein 4 homolog A
• Protein Family: Radial spoke head protein
• UniProt Gene Name: Rsph4a
• UniProt Synonym Gene Names: Rshl3
• UniProt Entry Name: RSH4A_MOUSE

Uniprot Description

RSHL3: Probable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules. Defects in RSPH4A are the cause of primary ciliary dyskinesia type 11 (CILD11). CILD is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. Belongs to the flagellar radial spoke RSP4/6 family. 3 isoforms of the human protein are produced by alternative splicing.

Cellular Component: nucleoplasm; cytoskeleton; cell projection; cytoplasm; nucleolus; nucleus; cilium

Biological Process: axoneme biogenesis