PHF20 sirna

PHF20 siRNA (Mouse)

Cat. Num. AAA8217166

• Gene Names: Phf20; 6820402O20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PHF20; PHF20 siRNA (Mouse); HCA58; PHD finger protein 20; Hepatocellular carcinoma-associated antigen 58 homolog; PHF20 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PHF20 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,004

• Applicable Applications for PHF20 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PHF20 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PHF20 sirna

siRNA to inhibit PHF20 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124487307
• NCBI GeneID: 228829
• NCBI Accession #: NP_766262.2
• NCBI GenBank Nucleotide #: NM_172674.2
• UniProt Accession #: Q8BLG0; Q8BMA2; Q8BYR4; Q921N1
• Molecular Weight: 114,284 Da
• NCBI Official Full Name: PHD finger protein 20
• NCBI Official Synonym Full Names: PHD finger protein 20
• NCBI Official Symbol: Phf20
• NCBI Official Synonym Symbols: 6820402O20Rik
• NCBI Protein Information: PHD finger protein 20
• UniProt Protein Name: PHD finger protein 20
• Protein Family: PHD finger protein
• UniProt Gene Name: Phf20
• UniProt Synonym Gene Names: Hca58
• UniProt Entry Name: PHF20_MOUSE

Uniprot Description

PHF20: Methyllysine-binding protein, component of the MOF histone acetyltransferase protein complex. Not required for maintaining the global histone H4 'Lys-16' acetylation (H4K16ac) levels or locus specific histone acetylation, but instead works downstream in transcriptional regulation of MOF target genes. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Acetyltransferase; DNA-binding; Nuclear envelope; C2H2-type zinc finger protein

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus; histone acetyltransferase complex

Molecular Function: DNA binding; zinc ion binding; metal ion binding; histone acetyltransferase activity (H4-K16 specific)

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification