MAP7 sirna

MAP7 siRNA (Human)

Cat. Num. AAA8216729

• Gene Names: MAP7; EMAP115; E-MAP-115
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAP7; MAP7 siRNA (Human); Ensconsin; Epithelial microtubule-associated protein of 115 kDa; E-MAP-115; Microtubule-associated protein 7; MAP-7; MAP7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MAP7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 771

• Applicable Applications for MAP7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAP7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAP7 sirna

siRNA to inhibit MAP7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 310750348
• NCBI GeneID: 9053
• NCBI Accession #: NP_001185537.1
• NCBI GenBank Nucleotide #: NM_001198608.1
• UniProt Accession #: Q14244; Q7Z6S0; Q8TAU5; Q9NY82; Q9NY83; B7Z290; B7Z400; B7Z5S7; B7Z9U7; C9JPS0; E9PCP3; F5H1E2
• Molecular Weight: 86,952 Da
• NCBI Official Full Name: ensconsin isoform 1
• NCBI Official Synonym Full Names: microtubule-associated protein 7
• NCBI Official Symbol: MAP7
• NCBI Official Synonym Symbols: EMAP115; E-MAP-115
• NCBI Protein Information: ensconsin
• UniProt Protein Name: Ensconsin
• Protein Family: MAP7 domain-containing protein
• UniProt Gene Name: MAP7
• UniProt Synonym Gene Names: E-MAP-115; MAP-7
• UniProt Entry Name: MAP7_HUMAN

NCBI Description

The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

MAP7: Microtubule-stabilizing protein that may play an important role during reorganization of microtubules during polarization and differentiation of epithelial cells. Associates with microtubules in a dynamic manner. May play a role in the formation of intercellular contacts. Colocalization with TRPV4 results in the redistribution of TRPV4 toward the membrane and may link cytoskeletal microfilaments. Belongs to the MAP7 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6q23.3

Cellular Component: microtubule cytoskeleton; microtubule; microtubule associated complex; basolateral plasma membrane; perinuclear region of cytoplasm; cytoplasm

Molecular Function: structural molecule activity; receptor binding

Biological Process: establishment and/or maintenance of cell polarity; microtubule cytoskeleton organization and biogenesis; response to osmotic stress