POM121 sirna

POM121 siRNA (Human)

Cat. Num. AAA8216564

• Gene Names: POM121; P145; POM121A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POM121; POM121 siRNA (Human); KIAA0618; NUP121; POM121A; Nuclear envelope pore membrane protein POM 121; Nuclear envelope pore membrane protein POM 121A; Nucleoporin Nup121; Pore membrane protein of 121 kDa; POM121 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POM121 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 999

• Applicable Applications for POM121 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POM121 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POM121 sirna

siRNA to inhibit POM121 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 380692334
• NCBI GeneID: 9883
• NCBI Accession #: NP_001244119.1
• NCBI GenBank Nucleotide #: NM_001257190.2
• UniProt Accession #: Q96HA1; O75115; Q96DI0; Q9H9X1; Q9Y2N3; Q9Y4S7; A6NFS9; A8CDT4; A8K933; A8MXF9
• Molecular Weight: 100,864 Da
• NCBI Official Full Name: nuclear envelope pore membrane protein POM 121 isoform 1
• NCBI Official Synonym Full Names: POM121 transmembrane nucleoporin
• NCBI Official Symbol: POM121
• NCBI Official Synonym Symbols: P145; POM121A
• NCBI Protein Information: nuclear envelope pore membrane protein POM 121
• UniProt Protein Name: Nuclear envelope pore membrane protein POM 121
• Protein Family: POM121-like protein
• UniProt Gene Name: POM121
• UniProt Synonym Gene Names: KIAA0618; NUP121; POM121A
• UniProt Entry Name: P121A_HUMAN

NCBI Description

This gene encodes a transmembrane protein that localizes to the inner nuclear membrane and forms a core component of the nuclear pore complex, which mediates transport to and from the nucleus. The encoded protein may anchor this complex to the nuclear envelope. There are multiple related genes and pseudogenes for this gene on chromosomes 5, 7, 15, and 22. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013]

Uniprot Description

POM121: a single-pass membrane protein that is an essential component of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. When overexpressed in cells induces the formation of cytoplasmic annulate lamellae (AL). Stably associated with the NPC throughout interphase and with the endoplasmic reticulum during metaphase. Contains F-X-F-G repeats. Belongs to the POM121 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleoporin; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; nuclear pore; nuclear envelope

Biological Process: mRNA transport; viral reproduction; cytokine and chemokine mediated signaling pathway; mitotic nuclear envelope disassembly; pathogenesis; glucose transport; viral infectious cycle; protein transport; hexose transport; carbohydrate metabolic process; viral transcription; gene expression; mitotic cell cycle; transmembrane transport