KLRC4 sirna

KLRC4 siRNA (Human)

Cat. Num. AAA8215966

• Gene Names: KLRC4; NKG2F; NKG2-F
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KLRC4; KLRC4 siRNA (Human); NKG2F; NKG2-F type II integral membrane protein; NK cell receptor F; NKG2-F-activating NK receptor; KLRC4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KLRC4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 158

• Applicable Applications for KLRC4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KLRC4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KLRC4 sirna

siRNA to inhibit KLRC4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7710124
• NCBI GeneID: 8302
• NCBI Accession #: NP_038459.1
• NCBI GenBank Nucleotide #: NM_013431.2
• UniProt Accession #: O43908; O60851
• Molecular Weight: 18,234 Da
• NCBI Official Full Name: NKG2-F type II integral membrane protein
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily C, member 4
• NCBI Official Symbol: KLRC4
• NCBI Official Synonym Symbols: NKG2F; NKG2-F
• NCBI Protein Information: NKG2-F type II integral membrane protein
• UniProt Protein Name: NKG2-F type II integral membrane protein
• Protein Family: NKG2-F type II integral membrane protein
• UniProt Gene Name: KLRC4
• UniProt Synonym Gene Names: NKG2F
• UniProt Entry Name: NKG2F_HUMAN

NCBI Description

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. This gene is a member of the NKG2 group of genes that are expressed primarily in natural killer (NK) cells. These family members encode transmembrane proteins that are characterized by a type II membrane orientation (have an extracellular C-terminus) and the presence of a C-type lectin domain. This family member is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. Read-through transcription exists between this gene and the downstream KLRK1 (killer cell lectin-like receptor subfamily K, member 1) family member. [provided by RefSeq, Dec 2010]

Uniprot Description

KLRC4: May play a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2-p12.3

Cellular Component: integral to membrane

Biological Process: cellular defense response