NOP10 sirna

NOP10 siRNA (Mouse)

Cat. Num. AAA8215960

• Gene Names: Nop10; Nola3; NOP10P; 1110036B12Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NOP10; NOP10 siRNA (Mouse); NOLA3; H/ACA ribonucleoprotein complex subunit 3; Nucleolar protein 10; Nucleolar protein family A member 3; snoRNP protein NOP10; NOP10 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NOP10 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 64

• Applicable Applications for NOP10 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NOP10 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NOP10 sirna

siRNA to inhibit NOP10 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13384790
• NCBI GeneID: 66181
• NCBI Accession #: NP_079679.1
• NCBI GenBank Nucleotide #: NM_025403.4
• UniProt Accession #: Q9CQS2
• Molecular Weight: 7,706 Da
• NCBI Official Full Name: H/ACA ribonucleoprotein complex subunit 3
• NCBI Official Synonym Full Names: NOP10 ribonucleoprotein
• NCBI Official Symbol: Nop10
• NCBI Official Synonym Symbols: Nola3; NOP10P; 1110036B12Rik
• NCBI Protein Information: H/ACA ribonucleoprotein complex subunit 3
• UniProt Protein Name: H/ACA ribonucleoprotein complex subunit 3
• Protein Family: Ribosome biogenesis protein
• UniProt Gene Name: Nop10
• UniProt Synonym Gene Names: Nola3
• UniProt Entry Name: NOP10_MOUSE

Uniprot Description

NOP10: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Defects in NOP10 are a cause of dyskeratosis congenita autosomal recessive type 1 (DKCB1). A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy. Belongs to the NOP10 family.

Protein type: Nucleolus

Cellular Component: nucleus; ribonucleoprotein complex

Molecular Function: snoRNA binding

Biological Process: pseudouridine synthesis; ribosome biogenesis and assembly; rRNA processing

Disease: Dyskeratosis Congenita, Autosomal Recessive, 1