ETS1 sirna

ETS1 siRNA (Rat)

Cat. Num. AAA8215884

• Gene Names: Ets1; Tpl1; Ets-1; Etsoncb
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ETS1; ETS1 siRNA (Rat); ETS-1; Protein C-ets-1; p54; ETS1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ETS1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 441

• Applicable Applications for ETS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ETS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ETS1 sirna

siRNA to inhibit ETS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978819
• NCBI GeneID: 24356
• NCBI Accession #: NP_036687.1
• NCBI GenBank Nucleotide #: NM_012555.2
• UniProt Accession #: P41156; Q3T1H1
• Molecular Weight: 50,423 Da
• NCBI Official Full Name: protein C-ets-1
• NCBI Official Synonym Full Names: v-ets avian erythroblastosis virus E26 oncogene homolog 1
• NCBI Official Symbol: Ets1
• NCBI Official Synonym Symbols: Tpl1; Ets-1; Etsoncb
• NCBI Protein Information: protein C-ets-1
• UniProt Protein Name: Protein C-ets-1
• Protein Family: Protein
• UniProt Gene Name: Ets1
• UniProt Synonym Gene Names: Ets-1
• UniProt Entry Name: ETS1_RAT

NCBI Description

activates transcription of cyclin-dependent kinase inhibitor p21WAF1/Cip1; plays a role in inhibition of apoptosis [RGD, Feb 2006]

Uniprot Description

Ets-1: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus oncogene. The DNA binding activity of Ets1 is controlled by kinases and transcription factors. It contributes to the regulation of cellular differentiation in hematopoietic cells. Ets1 promotes invasive behavior in endothelial cells, vascular smooth muscle cells and epithelial cancer cells. Regulates the expression of MMP1, MMP3, MMP9 and uPA as well as of VEGF and VEGFR gene expression. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; DNA-binding; Oncoprotein; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; intercellular bridge; cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; hypothalamus development; immune system process; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; cell motility involved in cell locomotion; PML body organization and biogenesis; female pregnancy; positive regulation of cell motility; angiogenesis involved in wound healing; regulation of angiogenesis; response to estradiol stimulus; negative regulation of cell cycle; regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; response to antibiotic; positive regulation of angiogenesis; response to mechanical stimulus; pituitary gland development; positive regulation of cell proliferation; response to wounding; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; cell differentiation; positive regulation of cell migration