LRRK2 sirna

LRRK2 siRNA (Mouse)

Cat. Num. AAA8214895

• Gene Names: Lrrk2; Gm927; cI-46; AW561911; 4921513O20Rik; 9330188B09Rik; D630001M17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LRRK2; LRRK2 siRNA (Mouse); Leucine-rich repeat serine/threonine-protein kinase 2; LRRK2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LRRK2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2527

• Applicable Applications for LRRK2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LRRK2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LRRK2 sirna

siRNA to inhibit LRRK2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 146231954
• NCBI GeneID: 66725
• NCBI Accession #: NP_080006.3
• NCBI GenBank Nucleotide #: NM_025730.3
• UniProt Accession #: Q5S006; Q8BWG7; Q8BZJ6; Q8CI84; Q8K062; E9QNJ2
• Molecular Weight: 284,732 Da
• NCBI Official Full Name: leucine-rich repeat serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: leucine-rich repeat kinase 2
• NCBI Official Symbol: Lrrk2
• NCBI Official Synonym Symbols: Gm927; cI-46; AW561911; 4921513O20Rik; 9330188B09Rik; D630001M17Rik
• NCBI Protein Information: leucine-rich repeat serine/threonine-protein kinase 2
• UniProt Protein Name: Leucine-rich repeat serine/threonine-protein kinase 2
• Protein Family: Leucine-rich repeat serine/threonine-protein kinase
• UniProt Gene Name: Lrrk2
• UniProt Entry Name: LRRK2_MOUSE

Uniprot Description

LRRK2: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). May play a role in the etiology of Parkinson disease. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Interacts with PARK2, PRDX3 and TPCN2. Expressed throughout the adult brain, but at a lower level than in heart and liver. Expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; TKL group; LRRK family

Cellular Component: dendrite cytoplasm; Golgi apparatus; extracellular space; mitochondrion; lysosome; dendrite; terminal button; inclusion body; trans-Golgi network; ribonucleoprotein complex; cytosol; lipid raft; synaptic vesicle; mitochondrial outer membrane; membrane; cell soma; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; synapse; cytoplasmic vesicle; intracellular; endosome; neuron projection; intracellular membrane-bound organelle; endoplasmic reticulum; perikaryon; cell projection; axon; mitochondrial membrane; plasma membrane; cell junction

Molecular Function: tubulin binding; GTPase activity; identical protein binding; GTP-dependent protein kinase activity; clathrin binding; protein homodimerization activity; Rho GTPase binding; GTP binding; nucleotide binding; actin binding; GTPase activator activity; protein kinase activity; transferase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; SNARE binding; transferase activity, transferring phosphorus-containing groups; protein kinase A binding; syntaxin-1 binding; kinase activity; glycoprotein binding; ATP binding

Biological Process: regulation of synaptic transmission, glutamatergic; activation of MAPKK activity; positive regulation of protein binding; activation of MAPK activity; protein amino acid autophosphorylation; regulation of neuron maturation; determination of adult life span; protein amino acid phosphorylation; tangential migration from the subventricular zone to the olfactory bulb; negative regulation of protein amino acid phosphorylation; regulation of dopamine receptor signaling pathway; small GTPase mediated signal transduction; cell differentiation; regulation of mitochondrial depolarization; negative regulation of protein binding; Golgi organization and biogenesis; neuromuscular junction development; positive regulation of autophagy; positive regulation of dopamine receptor signaling pathway; olfactory bulb development; peptidyl-threonine phosphorylation; MAPKKK cascade; endocytosis; regulation of locomotion; regulation of membrane potential; positive regulation of programmed cell death; peptidyl-serine phosphorylation; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; intracellular distribution of mitochondria; autophagy; positive regulation of protein amino acid phosphorylation; response to oxidative stress; regulation of excitatory postsynaptic membrane potential; phosphorylation; neurite morphogenesis; positive regulation of GTPase activity