ALX1 sirna

ALX1 siRNA (Rat)

Cat. Num. AAA8214808

• Gene Names: Alx1; Cart1; CART1X
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALX1; ALX1 siRNA (Rat); CART1; ALX homeobox protein 1; Cartilage homeoprotein 1; CART-1; ALX1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ALX1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 326

• Applicable Applications for ALX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ALX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALX1 sirna

siRNA to inhibit ALX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978601
• NCBI GeneID: 25401
• NCBI Accession #: NP_037053.1
• NCBI GenBank Nucleotide #: NM_012921.1
• UniProt Accession #: Q63087
• Molecular Weight: 31,638 Da
• NCBI Official Full Name: ALX homeobox protein 1
• NCBI Official Synonym Full Names: ALX homeobox 1
• NCBI Official Symbol: Alx1
• NCBI Official Synonym Symbols: Cart1; CART1X
• NCBI Protein Information: ALX homeobox protein 1
• UniProt Protein Name: ALX homeobox protein 1
• Protein Family: ALX homeobox protein
• UniProt Gene Name: Alx1
• UniProt Synonym Gene Names: CART-1
• UniProt Entry Name: ALX1_RAT

NCBI Description

homeodomain transcription factor that may play a role in chondrocyte differentiation [RGD, Feb 2006]

Uniprot Description

Cart1: Transcriptional activator that acts at a palindromic recognition sequence to enhance the activity of the SV40 and TK promoters. Functions as a repressor with the prolactin promoter in vivo. May play a role in chondrocyte differentiation and may also influence cervix development. Defects in ALX1 are the cause of frontonasal dysplasia type 3 (FND3). The term frontonasal dysplasia describes an array of abnormalities affecting the eyes, forehead and nose and linked to midfacial dysraphia. The clinical picture is highly variable. Major findings include true ocular hypertelorism; broadening of the nasal root; median facial cleft affecting the nose and/or upper lip and palate; unilateral or bilateral clefting of the alae nasi; lack of formation of the nasal tip; anterior cranium bifidum occultum; a V-shaped or widow's peak frontal hairline. Belongs to the paired homeobox family.

Protein type: DNA-binding

Cellular Component: nucleoplasm; Golgi apparatus; transcription factor complex; nucleus

Molecular Function: sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: anterior/posterior pattern formation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; palate development; skeletal development; mesenchymal cell development; embryonic skeletal morphogenesis; embryonic limb morphogenesis