MAGI1 sirna

MAGI1 siRNA (Human)

Cat. Num. AAA8214556

• Gene Names: MAGI1; AIP3; BAP1; WWP3; AIP-3; BAP-1; BAIAP1; MAGI-1; Magi1d; TNRC19
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAGI1; MAGI1 siRNA (Human); AIP3; BAIAP1; BAP1; TNRC19; Membrane-associated guanylate kinase. WW and PDZ domain-containing protein 1; Atrophin-1-interacting protein 3; AIP-3; BAI1-associated protein 1; BAP-1; Membrane-associated guanylate kinase inverted 1; MAGI-1; Trinucleotide repeat-containing gene 19 protein; WW domain-containing protein 3; WWP3; MAGI1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MAGI1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1462

• Applicable Applications for MAGI1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAGI1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAGI1 sirna

siRNA to inhibit MAGI1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 74272284
• NCBI GeneID: 9223
• NCBI Accession #: NP_001028229.1
• NCBI GenBank Nucleotide #: NM_001033057.1
• UniProt Accession #: Q96QZ7; O00309; O43863; O75085; Q96QZ8; Q96QZ9; A8K188
• Molecular Weight: 157,781 Da
• NCBI Official Full Name: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform c
• NCBI Official Synonym Full Names: membrane associated guanylate kinase, WW and PDZ domain containing 1
• NCBI Official Symbol: MAGI1
• NCBI Official Synonym Symbols: AIP3; BAP1; WWP3; AIP-3; BAP-1; BAIAP1; MAGI-1; Magi1d; TNRC19
• NCBI Protein Information: membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
• UniProt Protein Name: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1
• Protein Family: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein
• UniProt Gene Name: MAGI1
• UniProt Synonym Gene Names: AIP3; BAIAP1; BAP1; TNRC19; AIP-3; BAP-1; MAGI-1; WWP3
• UniProt Entry Name: MAGI1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the membrane-associated guanylate kinase homologue (MAGUK) family. MAGUK proteins participate in the assembly of multiprotein complexes on the inner surface of the plasma membrane at regions of cell-cell contact. The product of this gene may play a role as scaffolding protein at cell-cell junctions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

MAGI1: May play a role as scaffolding protein at cell-cell junctions. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nucleoplasm; tight junction; cell projection; cytoplasm; plasma membrane; intercellular junction; cell junction

Molecular Function: protein C-terminus binding; protein binding; protein complex scaffold; alpha-actinin binding; ATP binding

Biological Process: cell surface receptor linked signal transduction; protein complex assembly; cell adhesion