CARS2 sirna

CARS2 siRNA (Human)

Cat. Num. AAA8214551

• Gene Names: CARS2; cysRS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CARS2; CARS2 siRNA (Human); Probable cysteine--tRNA ligase mitochondrial; Cysteinyl-tRNA synthetase; CysRS; CARS2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CARS2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 564

• Applicable Applications for CARS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CARS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CARS2 sirna

siRNA to inhibit CARS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13375695
• NCBI GeneID: 79587
• NCBI Accession #: NP_078813.1
• NCBI GenBank Nucleotide #: NM_024537.3
• UniProt Accession #: Q9HA77; Q8NI84; Q96IV4
• Molecular Weight: 62,224 Da
• NCBI Official Full Name: probable cysteine--tRNA ligase, mitochondrial
• NCBI Official Synonym Full Names: cysteinyl-tRNA synthetase 2, mitochondrial (putative)
• NCBI Official Symbol: CARS2
• NCBI Official Synonym Symbols: cysRS
• NCBI Protein Information: probable cysteine--tRNA ligase, mitochondrial
• UniProt Protein Name: Probable cysteine--tRNA ligase, mitochondrial
• UniProt Gene Name: CARS2
• UniProt Synonym Gene Names: CysRS
• UniProt Entry Name: SYCM_HUMAN

NCBI Description

This gene encodes a putative member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of cysteine to tRNA molecules. A splice-site mutation in this gene has been associated with a novel progressive myoclonic epilepsy disease with similar symptoms to MERRF syndrome. [provided by RefSeq, Mar 2015]

Uniprot Description

CARS2: Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: Ligase; Translation; EC 6.1.1.16; Mitochondrial

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: mitochondrial matrix; cytosol

Molecular Function: cysteine-tRNA ligase activity; metal ion binding; ATP binding

Biological Process: tRNA aminoacylation for protein translation; cysteinyl-tRNA aminoacylation; gene expression