SHH sirna

SHH siRNA (Human)

Cat. Num. AAA8214465

• Gene Names: SHH; TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SHH; SHH siRNA (Human); Sonic hedgehog protein; HHG-1; SHH sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SHH siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 462

• Applicable Applications for SHH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SHH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SHH sirna

siRNA to inhibit SHH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4506939
• NCBI GeneID: 6469
• NCBI Accession #: NP_000184.1
• NCBI GenBank Nucleotide #: NM_000193.3
• UniProt Accession #: Q15465; Q75MC9; A4D247
• Molecular Weight: 49,607 Da
• NCBI Official Full Name: sonic hedgehog protein isoform 1 preproprotein
• NCBI Official Synonym Full Names: sonic hedgehog
• NCBI Official Symbol: SHH
• NCBI Official Synonym Symbols: TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
• NCBI Protein Information: sonic hedgehog protein
• UniProt Protein Name: Sonic hedgehog protein
• Protein Family: Sonic hedgehog protein
• UniProt Gene Name: SHH
• UniProt Synonym Gene Names: SHH
• UniProt Entry Name: SHH_HUMAN

NCBI Description

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

Uniprot Description

SHH: Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. Belongs to the hedgehog family.

Protein type: Cell cycle regulation; Oncoprotein; Motility/polarity/chemotaxis; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; extracellular region; plasma membrane; cytosol; nucleus; lipid raft

Molecular Function: peptidase activity; laminin-1 binding; morphogen activity; protein binding; glycosaminoglycan binding; zinc ion binding; patched binding; calcium ion binding; glycoprotein binding

Biological Process: prostate gland development; central nervous system development; heart development; embryonic skeletal development; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; CD4-positive or CD8-positive, alpha-beta T cell lineage commitment; lymphoid progenitor cell differentiation; Wnt receptor signaling pathway through beta-catenin; embryonic pattern specification; proteolysis; telencephalon regionalization; positive regulation of T cell differentiation in the thymus; cell-cell signaling; embryonic digestive tract morphogenesis; midbrain development; embryonic foregut morphogenesis; male genitalia development; ectoderm development; positive regulation of smoothened signaling pathway; positive regulation of oligodendrocyte differentiation; oligodendrocyte development; inner ear development; neural crest cell migration; activation of hh target transcription factor; hindbrain development; embryonic limb morphogenesis; positive regulation of neuroblast proliferation; striated muscle development; camera-type eye development; neuron fate commitment; myotube differentiation; intermediate filament organization; endocytosis; positive regulation of skeletal muscle development; negative thymic T cell selection; positive regulation of skeletal muscle cell proliferation; osteoblast development; patterning of blood vessels; branching morphogenesis of a tube; positive regulation of cell division; regulation of proteolysis; polarity specification of anterior/posterior axis; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; metanephros development; negative regulation of apoptosis; embryonic forelimb morphogenesis; axon guidance; granule cell precursor proliferation; ventral midline development; spinal cord dorsal/ventral patterning; cell fate specification; thalamus development; palate development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of striated muscle cell differentiation; embryonic hindlimb morphogenesis; Bergmann glial cell differentiation; negative regulation of T cell proliferation; positive regulation of cell proliferation; forebrain development; pancreas development; thyroid gland development; heart looping; vasculogenesis; positive regulation of Wnt receptor signaling pathway; negative regulation of cell migration; dorsoventral neural tube patterning; positive thymic T cell selection; smoothened signaling pathway; organ formation; hair follicle morphogenesis; intein-mediated protein splicing; thymus development; regulation of odontogenesis; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; androgen metabolic process; positive regulation of immature T cell proliferation in the thymus; spinal cord motor neuron differentiation; pattern specification process; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; formation of anatomical boundary; regulation of cell proliferation; odontogenesis of dentine-containing teeth; myoblast differentiation; limb bud formation; stem cell development; negative regulation of cell differentiation; embryonic development; dorsal/ventral pattern formation; positive regulation of protein import into nucleus; ureteric bud branching; establishment of cell polarity; neuroblast proliferation; hindgut morphogenesis; blood coagulation; cell development; positive regulation of alpha-beta T cell differentiation; negative regulation of alpha-beta T cell differentiation; lung development

Disease: Solitary Median Maxillary Central Incisor; Schizencephaly; Microphthalmia, Isolated, With Coloboma 5; Holoprosencephaly 3