DACH2 sirna

DACH2 siRNA (Mouse)

Cat. Num. AAA8213544

• Gene Names: Dach2; 9430028N04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DACH2; DACH2 siRNA (Mouse); Dachshund homolog 2; Dach2; DACH2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DACH2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 606

• Applicable Applications for DACH2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DACH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DACH2 sirna

siRNA to inhibit DACH2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 217035136
• NCBI GeneID: 93837
• NCBI Accession #: NP_001136042.1
• NCBI GenBank Nucleotide #: NM_001142570.1
• UniProt Accession #: Q925Q8; Q8BMA0
• Molecular Weight: 54,698 Da
• NCBI Official Full Name: dachshund homolog 2 isoform 1
• NCBI Official Synonym Full Names: dachshund 2 (Drosophila)
• NCBI Official Symbol: Dach2
• NCBI Official Synonym Symbols: 9430028N04Rik
• NCBI Protein Information: dachshund homolog 2
• UniProt Protein Name: Dachshund homolog 2
• Protein Family: Dachshund
• UniProt Gene Name: Dach2
• UniProt Synonym Gene Names: Dach2
• UniProt Entry Name: DACH2_MOUSE

Uniprot Description

DACH2: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain. Belongs to the DACH/dachshund family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: nucleus

Molecular Function: DNA binding

Biological Process: development of primary female sexual characteristics; regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development