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Western Blot (WB) (Western blot analysis of SGK223 (pY413) expression in U251MG (A), HeLa (B) whole cell lysates.)

Rabbit anti-Human SGK223 Polyclonal Antibody | anti-SGK223 antibody

Anti-SGK223 (pY413) Phospho Antibody

Gene Names
SGK223; PRAGMIN
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
SGK223; Polyclonal Antibody; Anti-SGK223 (pY413) Phospho Antibody; Tyrosine-protein kinase SgK223; Sugen kinase 223; anti-SGK223 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of SGK223 (pY413) protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
1406
Applicable Applications for anti-SGK223 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SGK223. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of SGK223 (pY413) expression in U251MG (A), HeLa (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of SGK223 (pY413) expression in U251MG (A), HeLa (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of SGK223 (pY413) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of SGK223 (pY413) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-SGK223 antibody
Rabbit polyclonal antibody to SGK223 (pY413)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
149,624 Da
NCBI Official Full Name
tyrosine-protein kinase SgK223
NCBI Official Symbol
SGK223
NCBI Official Synonym Symbols
PRAGMIN
NCBI Protein Information
tyrosine-protein kinase SgK223
UniProt Protein Name
Tyrosine-protein kinase SgK223
Protein Family
UniProt Gene Name
SGK223
UniProt Entry Name
SG223_HUMAN

NCBI Description

This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar 2014]

Uniprot Description

SGK223: a protein kinase of the NKF3 family.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; Motility/polarity/chemotaxis; EC 2.7.10.2; Other group; NKF3 family

Chromosomal Location of Human Ortholog: 8p23.1

Molecular Function: non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation

Research Articles on SGK223

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Product Notes

The SGK223 sgk223 (Catalog #AAA8213366) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SGK223 (pY413) Phospho Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SGK223 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the SGK223 sgk223 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SGK223, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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