PPP2R4 sirna

PPP2R4 siRNA (Human)

Cat. Num. AAA8213049

• Gene Names: PPP2R4; PP2A; PR53; PTPA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PPP2R4; PPP2R4 siRNA (Human); PTPA; Serine/threonine-protein phosphatase 2A activator; PP2A. subunit B'. PR53 isoform; Phosphotyrosyl phosphatase activator; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B'; PPP2R4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PPP2R4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 288

• Applicable Applications for PPP2R4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PPP2R4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PPP2R4 sirna

siRNA to inhibit PPP2R4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 301129290
• NCBI GeneID: 5524
• NCBI Accession #: NP_001180326.1
• NCBI GenBank Nucleotide #: NM_001193397.1
• UniProt Accession #: Q15257; Q15258; Q53GZ3; Q5TZQ2; Q9BUK1; Q9NNZ7; Q9NNZ8; Q9NNZ9; A2A347; A9IZU4; B4DXM4
• Molecular Weight: 31,859 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A activator isoform e
• NCBI Official Synonym Full Names: protein phosphatase 2A activator, regulatory subunit 4
• NCBI Official Symbol: PPP2R4
• NCBI Official Synonym Symbols: PP2A; PR53; PTPA
• NCBI Protein Information: serine/threonine-protein phosphatase 2A activator
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A activator
• UniProt Gene Name: PPP2R4
• UniProt Synonym Gene Names: PTPA; PTPA
• UniProt Entry Name: PTPA_HUMAN

NCBI Description

Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP2R4: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine- protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A. Associates with PP2A heterodimeric core enzyme PP2A(D), composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A). Interacts with PPP2CB. Widely expressed. Belongs to the PTPA-type PPIase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis; EC 5.2.1.8

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleoplasm; protein phosphatase type 2A complex; cytoplasm; nucleus

Molecular Function: peptidyl-prolyl cis-trans isomerase activity; protein homodimerization activity; protein phosphatase 2A binding; protein heterodimerization activity; ATPase activity; protein phosphatase type 2A regulator activity; protein tyrosine phosphatase activator activity; ATP binding; receptor binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of protein amino acid dephosphorylation; protein peptidyl-prolyl isomerization; metabolic process; positive regulation of apoptosis; mitotic spindle organization and biogenesis in nucleus; negative regulation of phosphoprotein phosphatase activity; regulation of phosphoprotein phosphatase activity; positive regulation of protein amino acid dephosphorylation