ABHD5 sirna

ABHD5 siRNA (Mouse)

Cat. Num. AAA8212766

• Gene Names: Abhd5; CDS; IECN5; NCIE2; CGI-58; 1300003D03Rik; 2010002J10Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABHD5; ABHD5 siRNA (Mouse); 1-acylglycerol-3-phosphate O-acyltransferase ABHD5; Abhydrolase domain-containing protein 5; Lipid droplet-binding protein CGI-58; Protein CGI-58; ABHD5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ABHD5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 351

• Applicable Applications for ABHD5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ABHD5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABHD5 sirna

siRNA to inhibit ABHD5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13385690
• NCBI GeneID: 67469
• NCBI Accession #: NP_080455.1
• NCBI GenBank Nucleotide #: NM_026179.2
• UniProt Accession #: Q9DBL9; Q9CTY3
• Molecular Weight: 21,846 Da
• NCBI Official Full Name: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5
• NCBI Official Synonym Full Names: abhydrolase domain containing 5
• NCBI Official Symbol: Abhd5
• NCBI Official Synonym Symbols: CDS; IECN5; NCIE2; CGI-58; 1300003D03Rik; 2010002J10Rik
• NCBI Protein Information: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5
• UniProt Protein Name: 1-acylglycerol-3-phosphate O-acyltransferase ABHD5
• Protein Family: 1-acylglycerol-3-phosphate O-acyltransferase
• UniProt Gene Name: Abhd5
• UniProt Synonym Gene Names: Protein CGI-58
• UniProt Entry Name: ABHD5_MOUSE

Uniprot Description

ABHD5: a lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Colocalized with PLIN and ADFP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues, and Chanarin-Dorfman syndrome (CDS), a triglyceride storage disease with impaired long-chain fatty acid oxidation and icthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons.

Protein type: Lipase; EC 2.3.1.51; Transferase

Cellular Component: intracellular membrane-bound organelle; cytoplasm; lipid particle; nucleus; cytosol

Molecular Function: transferase activity; triacylglycerol lipase activity; protein binding; transferase activity, transferring acyl groups; lysophosphatidic acid acyltransferase activity; 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phosphatidic acid biosynthetic process; positive regulation of lipoprotein lipase activity; lipid metabolic process; fatty acid metabolic process; cell differentiation; phospholipid biosynthetic process