HARS2 sirna

HARS2 siRNA (Mouse)

Cat. Num. AAA8211880

• Gene Names: Hars2; HO3; HARSR; Harsl; 4631412B19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HARS2; HARS2 siRNA (Mouse); HARSL; Probable histidine--tRNA ligase mitochondrial; Histidine--tRNA ligase-like; Histidyl-tRNA synthetase; HisRS; HARS2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HARS2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 505

• Applicable Applications for HARS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HARS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HARS2 sirna

siRNA to inhibit HARS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18079343
• NCBI GeneID: 70791
• NCBI Accession #: NP_542367.1
• NCBI GenBank Nucleotide #: NM_080636.2
• UniProt Accession #: Q99KK9
• Molecular Weight: 56,985 Da
• NCBI Official Full Name: probable histidine--tRNA ligase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: histidyl-tRNA synthetase 2, mitochondrial (putative)
• NCBI Official Symbol: Hars2
• NCBI Official Synonym Symbols: HO3; HARSR; Harsl; 4631412B19Rik
• NCBI Protein Information: probable histidine--tRNA ligase, mitochondrial
• UniProt Protein Name: Probable histidine--tRNA ligase, mitochondrial
• UniProt Gene Name: Hars2
• UniProt Synonym Gene Names: Harsl; HisRS
• UniProt Entry Name: SYHM_MOUSE

NCBI Description

This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of histidine to tRNA molecules. Mutations in a similar gene in human have been associated with Perrault syndrome 2 (PRLTS2). [provided by RefSeq, Mar 2015]

Uniprot Description

HARS2: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes share a bidirectional promoter. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial; Ligase; EC 6.1.1.21; Translation

Cellular Component: mitochondrion; cytoplasm

Molecular Function: histidine-tRNA ligase activity; aminoacyl-tRNA ligase activity; nucleotide binding; ATP binding; ligase activity

Biological Process: tRNA aminoacylation for protein translation; translation; histidyl-tRNA aminoacylation