DKC1 sirna

DKC1 siRNA (Mouse)

Cat. Num. AAA8211100

• Gene Names: Dkc1; BC068171
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DKC1; DKC1 siRNA (Mouse); H/ACA ribonucleoprotein complex subunit 4; Dyskerin; Nopp140-associated protein of 57 kDa; Nucleolar protein NAP57; Nucleolar protein family A member 4; snoRNP protein DKC1; DKC1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DKC1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 509

• Applicable Applications for DKC1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DKC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DKC1 sirna

siRNA to inhibit DKC1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 91064867
• NCBI GeneID: 245474
• NCBI Accession #: NP_001025478.1
• NCBI GenBank Nucleotide #: NM_001030307.2
• UniProt Accession #: Q9ESX5; Q3UWE5
• Molecular Weight: 57,402 Da
• NCBI Official Full Name: H/ACA ribonucleoprotein complex subunit 4
• NCBI Official Synonym Full Names: dyskeratosis congenita 1, dyskerin
• NCBI Official Symbol: Dkc1
• NCBI Official Synonym Symbols: BC068171
• NCBI Protein Information: H/ACA ribonucleoprotein complex subunit 4
• UniProt Protein Name: H/ACA ribonucleoprotein complex subunit 4
• UniProt Gene Name: Dkc1
• UniProt Entry Name: DKC1_MOUSE

Uniprot Description

dyskerin: Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Defects in DKC1 are a cause of dyskeratosis congenita X- linked recessive (XDKC). XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy. Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS). HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. Belongs to the pseudouridine synthase TruB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; RNA-binding; Nucleolus; EC 5.4.99.-; RNA processing; Isomerase

Cellular Component: nucleoplasm; Cajal body; small nucleolar ribonucleoprotein complex; telomerase holoenzyme complex; nucleolus; nucleus; ribonucleoprotein complex

Molecular Function: telomerase activity; beta-amyrin synthase activity; RNA binding; pseudouridine synthase activity; lupeol synthase activity; isomerase activity

Biological Process: pseudouridine synthesis; box H/ACA snoRNA metabolic process; rRNA pseudouridine synthesis; RNA-dependent DNA replication; snRNA pseudouridine synthesis; ribosome biogenesis and assembly; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; rRNA modification; RNA processing; cell proliferation; RNA modification; box H/ACA snoRNA 3'-end processing; rRNA processing