ZFP91 sirna

ZFP91 siRNA (Mouse)

Cat. Num. AAA8211011

• Gene Names: Zfp91; Pzf; Zfp-91; AL024263; AW545902; 9130014I08Rik; A530054C17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZFP91; ZFP91 siRNA (Mouse); PZF; E3 ubiquitin-protein ligase ZFP91; Penta Zf protein; Zinc finger protein 91 homolog; Zfp-91; Zinc finger protein PZF; ZFP91 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ZFP91 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 455

• Applicable Applications for ZFP91 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ZFP91 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZFP91 sirna

siRNA to inhibit ZFP91 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 90669983
• NCBI GeneID: 109910
• NCBI Accession #: NP_443735.2
• NCBI GenBank Nucleotide #: NM_053009.3
• UniProt Accession #: Q62511; Q3TEQ3; Q3UH61; Q62509; Q6P219; Q6PG06; Q8BPY3; Q8C2B4; Q8CDZ3
• Molecular Weight: 51,539 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase ZFP91
• NCBI Official Synonym Full Names: zinc finger protein 91
• NCBI Official Symbol: Zfp91
• NCBI Official Synonym Symbols: Pzf; Zfp-91; AL024263; AW545902; 9130014I08Rik; A530054C17Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase ZFP91
• UniProt Protein Name: E3 ubiquitin-protein ligase ZFP91
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Zfp91
• UniProt Synonym Gene Names: Pzf; Zfp-91
• UniProt Entry Name: ZFP91_MOUSE

NCBI Description

The protein encoded by this gene is a member of the zinc finger family of proteins. The gene product contains C2H2-type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. The homologous human protein has been shown to function as a regulator of the non-canonical NF-kappaB pathway in lymphotoxin-beta receptor signaling. A read-through transcript variant composed of Zfp91 and the downstream Cntf gene sequence has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. A Zfp91-related pseudogene has also been identified on chromosome 17. [provided by RefSeq, Oct 2010]

Uniprot Description

ZFP91: Atypical E3 ubiquitin-protein ligase that mediates 'Lys- 63'-linked ubiquitination of MAP3K14/NIK, leading to stabilize and activate MAP3K14/NIK. It thereby acts as an activator of the non- canonical NF-kappa-B2/NFKB2 pathway. May also play an important role in cell proliferation and/or anti-apoptosis. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; C2H2-type zinc finger protein

Cellular Component: nucleus

Molecular Function: nucleic acid binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: activation of NF-kappaB-inducing kinase