PTPRQ sirna

PTPRQ siRNA (Rat)

Cat. Num. AAA8210163

• Gene Names: Ptprq; rPTP-GMC1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PTPRQ; PTPRQ siRNA (Rat); PTPGMC1; Phosphatidylinositol phosphatase PTPRQ; Protein-tyrosine phosphatase receptor-type expressed by glomerular mesangial cells protein 1; rPTP-GMC1; Receptor-type tyrosine-protein phosphatase Q; PTP-RQ; R-PTP-Q; PTPRQ sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PTPRQ siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2302

• Applicable Applications for PTPRQ sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PTPRQ gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PTPRQ sirna

siRNA to inhibit PTPRQ expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 12621078
• NCBI GeneID: 360417
• NCBI Accession #: NP_075214.1
• NCBI GenBank Nucleotide #: NM_022925.1
• UniProt Accession #: O88488
• Molecular Weight: 256,824 Da
• NCBI Official Full Name: phosphatidylinositol phosphatase PTPRQ
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, Q
• NCBI Official Symbol: Ptprq
• NCBI Official Synonym Symbols: rPTP-GMC1
• NCBI Protein Information: phosphatidylinositol phosphatase PTPRQ
• UniProt Protein Name: Phosphatidylinositol phosphatase PTPRQ
• Protein Family: Phosphatidylinositol phosphatase
• UniProt Gene Name: Ptprq
• UniProt Synonym Gene Names: Ptpgmc1; rPTP-GMC1; PTP-RQ; R-PTP-Q
• UniProt Entry Name: PTPRQ_RAT

NCBI Description

may play a role in cell adhesion and migration [RGD, Feb 2006]

Uniprot Description

PTPRQ: Phosphatidylinositol phosphatase required for auditory function. May act by regulating the level of phosphatidylinositol 4,5-bisphosphate (PIP2) level in the basal region of hair bundles. Can dephosphorylate a broad range of phosphatidylinositol phosphates, including phosphatidylinositol 3,4,5-trisphosphate and most phosphatidylinositol monophosphates and diphosphates. Phosphate can be hydrolyzed from the D3 and D5 positions in the inositol ring. Has low tyrosine-protein phosphatase activity; however, the relevance of such activity in vivo is unclear. Plays an important role in adipogenesis of mesenchymal stem cells (MSCs). Regulates the phosphorylation state of AKT1 by suppressing the phosphatidylinositol 3,4,5-trisphosphate (PIP3) level in MSCs and preadipocyte cells. Defects in PTPRQ are the cause of deafness autosomal recessive type 84 (DFNB84). DFNB84 is a form of non- syndromic deafness characterized by progressive, sensorineural hearing loss and vestibular dysfunction. Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily.

Protein type: Hydrolase; Membrane protein, integral; Receptor, misc.; Protein phosphatase, tyrosine (non-receptor); Receptor protein phosphatase, tyrosine; EC 3.1.3.48; Phosphatase, lipid

Cellular Component: stereocilium bundle; plasma membrane; integral to membrane

Molecular Function: protein tyrosine phosphatase activity

Biological Process: vestibular receptor cell morphogenesis; inner ear morphogenesis; regulation of fat cell differentiation; hemopoietic progenitor cell differentiation; detection of mechanical stimulus involved in sensory perception of sound; neuromuscular process controlling balance