RGS9BP sirna

RGS9BP siRNA (Human)

Cat. Num. AAA8209474

• Gene Names: RGS9BP; R9AP; RGS9; PERRS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RGS9BP; RGS9BP siRNA (Human); R9AP; Regulator of G-protein signaling 9-binding protein; RGS9-anchoring protein; RGS9BP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RGS9BP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 235

• Applicable Applications for RGS9BP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RGS9BP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RGS9BP sirna

siRNA to inhibit RGS9BP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 115496700
• NCBI GeneID: 388531
• NCBI Accession #: NP_997274.2
• NCBI GenBank Nucleotide #: NM_207391.2
• UniProt Accession #: Q6ZS82; Q6ZVJ6
• Molecular Weight: 25,148 Da
• NCBI Official Full Name: regulator of G-protein signaling 9-binding protein
• NCBI Official Synonym Full Names: regulator of G protein signaling 9 binding protein
• NCBI Official Symbol: RGS9BP
• NCBI Official Synonym Symbols: R9AP; RGS9; PERRS
• NCBI Protein Information: regulator of G-protein signaling 9-binding protein
• UniProt Protein Name: Regulator of G-protein signaling 9-binding protein
• Protein Family: Regulator of G-protein signaling 9-binding protein
• UniProt Gene Name: RGS9BP
• UniProt Synonym Gene Names: R9AP
• UniProt Entry Name: R9BP_HUMAN

NCBI Description

The protein encoded by this gene functions as a regulator of G protein-coupled receptor signaling in phototransduction. Studies in bovine and mouse show that this gene is expressed only in the retina, and is localized in the rod outer segment membranes. This protein is associated with a heterotetrameric complex, specifically interacting with the regulator of G-protein signaling 9, and appears to function as the membrane anchor for the other largely soluble interacting partners. Mutations in this gene are associated with prolonged electroretinal response suppression (PERRS), also known as bradyopsia. [provided by RefSeq, Mar 2010]

Uniprot Description

RGS9BP: Regulator of G protein-coupled receptor (GPCR) signaling in phototransduction. Participates in the recovery phase of visual transduction via its interaction with RGS9-1 isoform. Acts as a membrane-anchor that mediates the targeting of RGS9-1 to the photoreceptor outer segment, where phototransduction takes place. Enhances the ability of RGS9-1 to stimulate G protein GTPase activity, allowing the visual signal to be terminated on the physiologically time scale. It also controls the proteolytic stability of RGS9-1, probably by protecting it from degradation. Defects in RGS9BP are a cause of prolonged electroretinal response suppression (PERRS); also known as bradyopsia. PERRS is characterized by difficulty adjusting to sudden changes in luminance levels mediated by cones. Belongs to the RGS7BP/RGS9BP family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: integral to membrane

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; detection of light stimulus involved in visual perception; negative regulation of signal transduction

Disease: Prolonged Electroretinal Response Suppression