PRRT2 sirna

PRRT2 siRNA (Rat)

Cat. Num. AAA8209070

• Gene Names: Prrt2; DSPB3; RGD1564195
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRRT2; PRRT2 siRNA (Rat); Proline-rich transmembrane protein 2; Dispanin subfamily B member 3; DSPB3; PRRT2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PRRT2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 344

• Applicable Applications for PRRT2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PRRT2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRRT2 sirna

siRNA to inhibit PRRT2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 451770387
• NCBI GeneID: 361651
• NCBI Accession #: NP_001263399.1
• NCBI GenBank Nucleotide #: NM_001276470.1
• UniProt Accession #: D3ZFB6
• Molecular Weight: 35,821 Da
• NCBI Official Full Name: proline-rich transmembrane protein 2
• NCBI Official Synonym Full Names: proline-rich transmembrane protein 2
• NCBI Official Symbol: Prrt2
• NCBI Official Synonym Symbols: DSPB3; RGD1564195
• NCBI Protein Information: proline-rich transmembrane protein 2
• UniProt Protein Name: Proline-rich transmembrane protein 2
• Protein Family: Proline-rich transmembrane protein
• UniProt Gene Name: Prrt2
• UniProt Synonym Gene Names: DSPB3
• UniProt Entry Name: PRRT2_RAT

Uniprot Description

PRRT2: Defects in PRRT2 are the cause of episodic kinesigenic dyskinesia type 1 (EKD1). An autosomal dominant neurologic condition characterized by recurrent and brief attacks of abnormal involuntary movements, triggered by sudden voluntary movement. These attacks usually have onset during childhood or early adulthood and can involve dystonic postures, chorea, or athetosis. Disease-causing mutations that produce truncation of the C-terminus of the protein alter subcellular location, from plasma membrane to cytosplasm (PubMed:22101681). Defects in PRRT2 are the cause of convulsions, familial infantile, with paroxysmal choreoathetosis (ICCA). A syndrome characterized by clinical features of benign familial infantile seizures and episodic kinesigenic dyskinesia. Benign familial infantile seizures is a disorder characterized by afebrile seizures occurring during the first year of life, without neurologic sequelae. Paroxysmal choreoathetosis is a disorder of involuntary movements characterized by attacks that occur spontaneously or are induced by a variety of stimuli. Defects in PRRT2 are the cause of seizures, benign familial infantile type 2 (BFIS2). An autosomal dominant disorder in which afebrile seizures occur in clusters during the first year of life, without neurologic sequelae. Belongs to the CD225/Dispanin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: membrane; integral to membrane; plasma membrane; synapse; cell junction

Biological Process: neuromuscular process controlling posture