ADAM2 sirna

ADAM2 siRNA (Mouse)

Cat. Num. AAA8209000

• Gene Names: Adam2; Ftnb; AI323749; Ph30-beta
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADAM2; ADAM2 siRNA (Mouse); FTNB; Disintegrin and metalloproteinase domain-containing protein 2; ADAM 2; Fertilin subunit beta; PH-30; PH30; PH30-beta; ADAM2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ADAM2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 735

• Applicable Applications for ADAM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ADAM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADAM2 sirna

siRNA to inhibit ADAM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 116089327
• NCBI GeneID: 11495
• NCBI Accession #: NP_033748.2
• NCBI GenBank Nucleotide #: NM_009618.2
• UniProt Accession #: Q60718; Q60814; Q9D4G3; Q9QWJ0
• Molecular Weight: 82,375 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 2
• NCBI Official Synonym Full Names: a disintegrin and metallopeptidase domain 2
• NCBI Official Symbol: Adam2
• NCBI Official Synonym Symbols: Ftnb; AI323749; Ph30-beta
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 2
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 2
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam2
• UniProt Synonym Gene Names: Ftnb; ADAM 2; PH30
• UniProt Entry Name: ADAM2_MOUSE

Uniprot Description

ADAM2: a member of the ADAM (a disintegrin and metalloprotease domain) family of Cancer-Testis Antigen (CTAs) . CTAs may play roles in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovary cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins. This member is a subunit of an integral sperm membrane glycoprotein called fertilin, which plays an important role in sperm-egg interactions. A sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein. Two isoforms of the human protein are produced by alternative splicing. Note: This description may include information from RefSeq and UniProtKB

Protein type: Cell surface; Motility/polarity/chemotaxis; Membrane protein, integral; Cancer Testis Antigen (CTA)

Cellular Component: cell surface; membrane; acrosome; integral to membrane

Molecular Function: protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity

Biological Process: adult behavior; visual learning; proteolysis; cell adhesion