MYOCD sirna

MYOCD siRNA (Mouse)

Cat. Num. AAA8208811

• Gene Names: Myocd; Srfcp; BSAC2A
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYOCD; MYOCD siRNA (Mouse); BSAC2; MYCD; SRFCP; Myocardin; Basic SAP coiled-coil transcription activator 2; SRF cofactor protein; MYOCD sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MYOCD siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 983

• Applicable Applications for MYOCD sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MYOCD gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYOCD sirna

siRNA to inhibit MYOCD expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 154240675
• NCBI GeneID: 214384
• NCBI Accession #: NP_660118.3
• NCBI GenBank Nucleotide #: NM_145136.4
• UniProt Accession #: Q8VIM5; Q5SS65; Q6W8X1; Q8C3W6; Q8VIL4
• Molecular Weight: 92,243 Da
• NCBI Official Full Name: myocardin isoform A
• NCBI Official Synonym Full Names: myocardin
• NCBI Official Symbol: Myocd
• NCBI Official Synonym Symbols: Srfcp; BSAC2A
• NCBI Protein Information: myocardin
• UniProt Protein Name: Myocardin
• Protein Family: Myocardin
• UniProt Gene Name: Myocd
• UniProt Synonym Gene Names: Bsac2; Mycd; Srfcp
• UniProt Entry Name: MYCD_MOUSE

Uniprot Description

myocardin: Smooth muscle cells (SM) and cardiac muscle cells- specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Cell development/differentiation

Cellular Component: nucleus

Molecular Function: histone acetyltransferase binding; protein binding; histone deacetylase binding; transcription coactivator activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; regulation of cell growth by extracellular stimulus; heart development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; uterus development; cardiac muscle cell differentiation; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; regulation of smooth muscle cell differentiation; positive regulation of cell proliferation; positive regulation of smooth muscle contraction; vasculogenesis; positive regulation of DNA binding; transcription, DNA-dependent; urinary bladder development; positive regulation of smooth muscle cell differentiation; negative regulation of cyclin-dependent protein kinase activity; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of histone acetylation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; gut development; regulation of myoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; positive regulation of transcription factor activity; alveolus development