TPR sirna

TPR siRNA (Mouse)

Cat. Num. AAA8208257

• Gene Names: Tpr; C77892; 2610029M07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TPR; TPR siRNA (Mouse); Nucleoprotein TPR; NPC-associated intranuclear protein; Translocated promoter region and nuclear basket protein; TPR sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TPR siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2431

• Applicable Applications for TPR sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TPR gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TPR sirna

siRNA to inhibit TPR expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 270309140
• NCBI GeneID: 108989
• NCBI Accession #: NP_598541.3
• NCBI GenBank Nucleotide #: NM_133780.3
• UniProt Accession #: F6ZDS4; Q8R4A0; Q921B9
• Molecular Weight: 273,990 Da
• NCBI Official Full Name: nucleoprotein TPR
• NCBI Official Synonym Full Names: translocated promoter region, nuclear basket protein
• NCBI Official Symbol: Tpr
• NCBI Official Synonym Symbols: C77892; 2610029M07Rik
• NCBI Protein Information: nucleoprotein TPR
• UniProt Protein Name: Nucleoprotein TPR
• Protein Family: Thiol protease
• UniProt Gene Name: Tpr
• UniProt Entry Name: TPR_MOUSE

Uniprot Description

TPR: a large coiled-coil nuclear pore protein that forms filamentous structures extending from nuclear pore complexes (NPCs) into the perinucleolar region. Together with Lamina-associated polypeptide 2 (LAP2) family members and Nup153, forms structural links between the nuclear lamina and the internal nuclear matrix. In interphase, localizes to the nucleoplasmic side of the nuclear pore complex (NPC) core structure, forming a fibrous structure called the nuclear basket. Essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus. Involved in the formation and/or maintenance of NPC-associated perinuclear heterochromatin exclusion zones (HEZs). Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP70 promoter and with mRNAs transcribed from this promoter under stress-induced conditions. Translocations between human TPR and TrkA, MET, and RAF genes are oncogenic. Its N-terminus is involved in activation of oncogenic kinases.

Protein type: Membrane protein, peripheral; Nucleoporin; Oncoprotein; Nuclear envelope

Cellular Component: kinetochore; nuclear membrane; extrinsic to membrane; cytoskeleton; membrane; cytoplasmic dynein complex; cytoplasm; nuclear pore; nuclear envelope; chromosome; nucleus; chromosome, pericentric region; nuclear inclusion body

Molecular Function: tubulin binding; mRNA binding; protein binding; protein homodimerization activity; heat shock protein binding; mitogen-activated protein kinase binding; chromatin binding; nucleocytoplasmic transporter activity

Biological Process: mitosis; negative regulation of translational initiation; mRNA transport; positive regulation of heterochromatin formation; RNA export from nucleus; negative regulation of transcription from RNA polymerase II promoter; cell cycle; negative regulation of RNA export from nucleus; RNA import into nucleus; positive regulation of protein export from nucleus; protein transport; nuclear translocation of MAPK; mRNA export from nucleus during heat stress; positive regulation of protein import into nucleus; cell division; transport; protein import into nucleus; mitotic cell cycle spindle assembly checkpoint