SLC1A3 sirna

SLC1A3 siRNA (Mouse)

Cat. Num. AAA8208068

• Gene Names: Slc1a3; Gmt1; Eaat1; GLAST; GLU-T; GluT-1; MGluT1; GLAST-1; AI504299; B430115D02Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC1A3; SLC1A3 siRNA (Mouse); EAAT1; GMT1; Excitatory amino acid transporter 1; Glial high affinity glutamate transporter; High-affinity neuronal glutamate transporter; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3; SLC1A3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SLC1A3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 543

• Applicable Applications for SLC1A3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC1A3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC1A3 sirna

siRNA to inhibit SLC1A3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 24233554
• NCBI GeneID: 20512
• NCBI Accession #: NP_683740.1
• NCBI GenBank Nucleotide #: NM_148938.3
• UniProt Accession #: P56564; Q99P53
• Molecular Weight: 59,622 Da
• NCBI Official Full Name: excitatory amino acid transporter 1
• NCBI Official Synonym Full Names: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• NCBI Official Symbol: Slc1a3
• NCBI Official Synonym Symbols: Gmt1; Eaat1; GLAST; GLU-T; GluT-1; MGluT1; GLAST-1; AI504299; B430115D02Rik
• NCBI Protein Information: excitatory amino acid transporter 1
• UniProt Protein Name: Excitatory amino acid transporter 1
• Protein Family: Excitatory amino acid transporter
• UniProt Gene Name: Slc1a3
• UniProt Synonym Gene Names: Eaat1; Gmt1; GLAST-1
• UniProt Entry Name: EAA1_MOUSE

Uniprot Description

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Transporter; Transporter, SLC family

Cellular Component: neuron projection; cell projection; cell surface; cell soma; membrane; basolateral plasma membrane; mitochondrial inner membrane; fibril; dendritic spine; plasma membrane; integral to membrane; synapse

Molecular Function: amino acid binding; protein binding; glutamate binding; symporter activity; high-affinity glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; response to light stimulus; glutamate metabolic process; response to inorganic substance; L-glutamate transport; auditory behavior; cranial nerve development; glutamate biosynthetic process; response to amino acid stimulus; malate-aspartate shuttle; positive regulation of synaptic transmission; response to antibiotic; L-glutamate import; sensory perception of sound; transport; dicarboxylic acid transport; neuron morphogenesis during differentiation; gamma-aminobutyric acid biosynthetic process; response to wounding; neuromuscular process controlling balance