HSP90B1 sirna

HSP90B1 siRNA (Mouse)

Cat. Num. AAA8207915

• Gene Names: Hsp90b1; TA-3; Tra1; gp96; ERp99; GRP94; Targ2; Tra-1; endoplasmin
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HSP90B1; HSP90B1 siRNA (Mouse); GRP94; TRA-1; TRA1; Endoplasmin; 94 kDa glucose-regulated protein; GRP-94; Endoplasmic reticulum resident protein 99; ERp99; Heat shock protein 90 kDa beta member 1; Polymorphic tumor rejection antigen 1; Tumor rejection antigen gp96; HSP90B1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: HSP90B1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 802

• Applicable Applications for HSP90B1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse HSP90B1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HSP90B1 sirna

siRNA to inhibit HSP90B1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6755863
• NCBI GeneID: 22027
• NCBI Accession #: NP_035761.1
• NCBI GenBank Nucleotide #: NM_011631.1
• UniProt Accession #: P08113; P11427
• Molecular Weight: 92,476 Da
• NCBI Official Full Name: endoplasmin
• NCBI Official Synonym Full Names: heat shock protein 90, beta (Grp94), member 1
• NCBI Official Symbol: Hsp90b1
• NCBI Official Synonym Symbols: TA-3; Tra1; gp96; ERp99; GRP94; Targ2; Tra-1; endoplasmin
• NCBI Protein Information: endoplasmin
• UniProt Protein Name: Endoplasmin
• Protein Family: Endoplasmin
• UniProt Gene Name: Hsp90b1
• UniProt Synonym Gene Names: Grp94; Tra-1; Tra1; GRP-94; ERp99
• UniProt Entry Name: ENPL_MOUSE

Uniprot Description

GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.

Protein type: Heat shock protein; Apoptosis; Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; endoplasmic reticulum membrane; smooth endoplasmic reticulum; focal adhesion; membrane; endoplasmic reticulum lumen; perinuclear region of cytoplasm; endoplasmic reticulum; plasma membrane; midbody; cytosol; nucleus

Molecular Function: protein binding; low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; nucleotide binding; protein phosphatase binding; ATP binding

Biological Process: actin rod formation; ER-associated protein catabolic process; protein folding; response to hypoxia; response to stress; regulation of phosphoprotein phosphatase activity; negative regulation of apoptosis