AKR1C21 sirna

AKR1C21 siRNA (Mouse)

Cat. Num. AAA8207726

• Gene Names: Akr1c21; AI315367; 9430025F20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AKR1C21; AKR1C21 siRNA (Mouse); Aldo-keto reductase family 1 member C21; 17-alpha-hydroxysteroid dehydrogenase; 17-alpha-HSD; 3(or 17)-alpha-hydroxysteroid dehydrogenase; 3-alpha-hydroxysteroid dehydrogenase; Dihydrodiol dehydrogenase type 1; DD1; Dihydrodiol dehydrogenase type 3; DD3; AKR1C21 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AKR1C21 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 323

• Applicable Applications for AKR1C21 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AKR1C21 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AKR1C21 sirna

siRNA to inhibit AKR1C21 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 126090770
• NCBI GeneID: 77337
• NCBI Accession #: NP_084177.2
• NCBI GenBank Nucleotide #: NM_029901.2
• UniProt Accession #: Q91WR5; Q6P8V0; Q9CX32
• Molecular Weight: 36,877 Da
• NCBI Official Full Name: aldo-keto reductase family 1 member C21
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C21
• NCBI Official Symbol: Akr1c21
• NCBI Official Synonym Symbols: AI315367; 9430025F20Rik
• NCBI Protein Information: aldo-keto reductase family 1 member C21
• UniProt Protein Name: Aldo-keto reductase family 1 member C21
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: Akr1c21
• UniProt Synonym Gene Names: 17-alpha-HSD; DD1; DD3
• UniProt Entry Name: AK1CL_MOUSE

Uniprot Description

AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.3.1.20; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.357

Cellular Component: cytoplasm; cytosol

Molecular Function: mevaldate reductase activity; bile acid binding; oxidoreductase activity; C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity; phenanthrene 9,10-monooxygenase activity; 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; (R)-2-hydroxyisocaproate dehydrogenase activity; steroid dehydrogenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; carboxylic acid binding; D-arabinitol dehydrogenase (NADP+) activity; aldehyde reductase activity; acetoin dehydrogenase activity; gluconate dehydrogenase activity; (R)-2-hydroxyglutarate dehydrogenase activity; phenylcoumaran benzylic ether reductase activity; aldo-keto reductase activity; arabinose reductase activity; isocitrate dehydrogenase activity; epoxide dehydrogenase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; 3-alpha-hydroxysteroid dehydrogenase (B-specific) activity; 3-ketoglucose-reductase activity; steroid dehydrogenase activity, acting on the CH-CH group of donors; 2-hydroxytetrahydrofuran dehydrogenase activity; 5-exo-hydroxycamphor dehydrogenase activity; steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; xylose reductase activity

Biological Process: steroid metabolic process; lipid metabolic process; steroid biosynthetic process