WIPI2 sirna

WIPI2 siRNA (Human)

Cat. Num. AAA8207190

• Gene Names: WIPI2; Atg21; ATG18B; CGI-50; WIPI-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WIPI2; WIPI2 siRNA (Human); WD repeat domain phosphoinositide-interacting protein 2; WIPI-2; WIPI49-like protein 2; WIPI2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WIPI2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 443

• Applicable Applications for WIPI2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WIPI2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WIPI2 sirna

siRNA to inhibit WIPI2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 75677329
• NCBI GeneID: 26100
• NCBI Accession #: NP_001028690.1
• NCBI GenBank Nucleotide #: NM_001033518.1
• UniProt Accession #: Q9Y4P8; Q5MNZ8; Q6FI96; Q75L50; Q96IE4; Q9Y364; B3KNC2
• Molecular Weight: 48,249 Da
• NCBI Official Full Name: WD repeat domain phosphoinositide-interacting protein 2 isoform c
• NCBI Official Synonym Full Names: WD repeat domain, phosphoinositide interacting 2
• NCBI Official Symbol: WIPI2
• NCBI Official Synonym Symbols: Atg21; ATG18B; CGI-50; WIPI-2
• NCBI Protein Information: WD repeat domain phosphoinositide-interacting protein 2
• UniProt Protein Name: WD repeat domain phosphoinositide-interacting protein 2
• Protein Family: WD repeat domain phosphoinositide-interacting protein
• UniProt Gene Name: WIPI2
• UniProt Synonym Gene Names: WIPI-2
• UniProt Entry Name: WIPI2_HUMAN

NCBI Description

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]

Uniprot Description

WIPI2: Probable early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to PtdIns3P. May bind PtdIns3P. Interacts with TECPR1. Ubiquitously expressed. Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors. Belongs to the WD repeat SVP1 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: nucleoplasm; extrinsic to membrane; protein complex; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; cytosol

Molecular Function: protein binding; phosphatidylinositol 3-phosphate binding

Biological Process: autophagic vacuole fusion; protein amino acid lipidation; mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation