MUC5B sirna

MUC5B siRNA (Human)

Cat. Num. AAA8206661

• Gene Names: MUC5B; MG1; MUC5; MUC9; MUC-5B
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MUC5B; MUC5B siRNA (Human); MUC5; Mucin-5B; MUC-5B; Cervical mucin; High molecular weight salivary mucin MG1; Mucin-5 subtype B. tracheobronchial; Sublingual gland mucin; MUC5B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MUC5B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 5,762

• Applicable Applications for MUC5B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MUC5B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MUC5B sirna

siRNA to inhibit MUC5B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 301172750
• NCBI GeneID: 727897
• NCBI Accession #: NP_002449.2
• NCBI GenBank Nucleotide #: NM_002458.2
• UniProt Accession #: Q9HC84; O00447; O00573; O14985; O15494; O95291; O95451; Q14881; Q7M4S5; Q99552; Q9UE28
• Molecular Weight: 596,340 Da
• NCBI Official Full Name: mucin-5B
• NCBI Official Synonym Full Names: mucin 5B, oligomeric mucus/gel-forming
• NCBI Official Symbol: MUC5B
• NCBI Official Synonym Symbols: MG1; MUC5; MUC9; MUC-5B
• NCBI Protein Information: mucin-5B
• UniProt Protein Name: Mucin-5B
• Protein Family: Mucin
• UniProt Gene Name: MUC5B
• UniProt Synonym Gene Names: MUC5; MUC-5B
• UniProt Entry Name: MUC5B_HUMAN

NCBI Description

This gene encodes a member of the mucin family of proteins, which are highly glycosylated macromolecular components of mucus secretions. This family member is the major gel-forming mucin in mucus. It is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. This gene has been found to be up-regulated in some human diseases, including sinus mucosa of chronic rhinosinusitis (CRS), CRS with nasal polyposis, chronic obstructive pulmonary disease (COPD) and H. pylori-associated gastric disease, and it may be involved in the pathogenesis of these diseases. [provided by RefSeq, Jul 2010]

Uniprot Description

MUC5B: Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. Defects in MUC5B are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. A common polymorphism in the promoter of MUC5B is associated with familial interstitial pneumonia and idiopathic pulmonary fibrosis, suggesting that dysregulated MUC5B expression in the lung may be involved in the pathogenesis of pulmonary fibrosis (PubMed:21506741).

Protein type: Secreted, signal peptide; Cell adhesion; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; regulation of macrophage activation; defense response to bacterium; post-translational protein modification

Disease: Pulmonary Fibrosis, Idiopathic