ST13 sirna

ST13 siRNA (Human)

Cat. Num. AAA8206054

• Gene Names: ST13; HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ST13; ST13 siRNA (Human); AAG2; FAM10A1; HIP; SNC6; Hsc70-interacting protein; Hip; Aging-associated protein 2; Progesterone receptor-associated p48 protein; Protein FAM10A1; Putative tumor suppressor ST13; Renal carcinoma antigen NY-REN-33; Suppression of tumorigenicity 13 protein; ST13 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ST13 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 369

• Applicable Applications for ST13 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ST13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ST13 sirna

siRNA to inhibit ST13 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923193
• NCBI GeneID: 6767
• NCBI Accession #: NP_003923.2
• NCBI GenBank Nucleotide #: NM_003932.4
• UniProt Accession #: P50502; O14999; Q2TU77
• Molecular Weight: 41,332 Da
• NCBI Official Full Name: hsc70-interacting protein isoform 1
• NCBI Official Synonym Full Names: suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
• NCBI Official Symbol: ST13
• NCBI Official Synonym Symbols: HIP; HOP; P48; AAG2; SNC6; HSPABP; FAM10A1; FAM10A4; HSPABP1; PRO0786
• NCBI Protein Information: hsc70-interacting protein
• UniProt Protein Name: Hsc70-interacting protein
• Protein Family: Hsc70-interacting protein
• UniProt Gene Name: ST13
• UniProt Synonym Gene Names: AAG2; FAM10A1; HIP; SNC6; Hip
• UniProt Entry Name: F10A1_HUMAN

NCBI Description

The protein encoded by this gene is an adaptor protein that mediates the association of the heat shock proteins HSP70 and HSP90. This protein has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. The expression of this gene is reported to be downregulated in colorectal carcinoma tissue suggesting that it is a candidate tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]

Uniprot Description

HIP: One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins. Homotetramer. Interacts with HSC70 as well as DNAJ homologs and HSP90. Interacts (via the C-terminus 303- 319 AA) with GRK5. Belongs to the FAM10 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: protein complex; cytoplasm; cytosol

Molecular Function: protein binding, bridging; protein domain specific binding; identical protein binding; protein binding; chaperone binding; unfolded protein binding; dATP binding; protein complex binding; Hsp70 protein binding

Biological Process: protein folding; protein homooligomerization