Rabbit JIP3 Polyclonal Antibody | anti-MAPK8IP3 antibody

Anti-JIP3 Antibody

Cat. Num. AAA820556

• Gene Names: MAPK8IP3; syd; JIP3; SYD2; JSAP1
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: JIP3; Polyclonal Antibody; Anti-JIP3 Antibody; KIAA1066; C-Jun-amino-terminal kinase-interacting protein 3; JIP-3; JNK-interacting protein 3; JNK MAP kinase scaffold protein 3; Mitogen-activated protein kinase 8-interacting protein 3; anti-MAPK8IP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of JIP3 protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 1330

• Applicable Applications for anti-MAPK8IP3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human JIP3. The exact sequence is proprietary.
• Preparation and Storage: Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of JIP3 expression in BV2 (A), HuT78 (B), Hela (C), HEK293T (D) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of JIP3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunofluorescence (IF)

(Immunofluorescent analysis of JIP3 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

Related Product Information for anti-MAPK8IP3 antibody

Rabbit polyclonal antibody to JIP3

NCBI and Uniprot Product Information

• NCBI GI #: 94557295
• NCBI GeneID: 23162
• NCBI Accession #: NP_001035529.1
• NCBI GenBank Nucleotide #: NM_001040439.1
• UniProt Accession #: Q9UPT6; Q96RY4; Q9H4I4; Q9H7P1; Q9NUG0; A2A2B3; A7E2B3
• NCBI Official Full Name: C-Jun-amino-terminal kinase-interacting protein 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8 interacting protein 3
• NCBI Official Symbol: MAPK8IP3
• NCBI Official Synonym Symbols: syd; JIP3; SYD2; JSAP1
• NCBI Protein Information: C-Jun-amino-terminal kinase-interacting protein 3; JIP-3; JNK-interacting protein 3; JNK/SAPK-associated protein-1; JNK MAP kinase scaffold protein 3; homolog of Drosophila Sunday driver 2; C-jun-amino-terminal kinase interacting protein 3; JNK/stress-activated protein kinase-associated protein 1; mitogen-activated protein kinase 8-interacting protein 3
• UniProt Protein Name: C-Jun-amino-terminal kinase-interacting protein 3
• Protein Family: C-Jun-amino-terminal kinase-interacting protein
• UniProt Gene Name: MAPK8IP3
• UniProt Synonym Gene Names: JIP3; KIAA1066; JIP-3; JNK-interacting protein 3
• UniProt Entry Name: JIP3_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies of the similar gene in mouse suggested that this protein may interact with, and regulate the activity of numerous protein kinases of the JNK signaling pathway, and thus function as a scaffold protein in neuronal cells. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

JIP3: a member of the JNK-interacting protein group of scaffold proteins that selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Shares similarity with the product of Drosophila syd gene, required for the functional interaction of kinesin I with axonal cargo. Studies in the mouse suggested that this protein may interact with and regulate the activity of numerous protein kinases of the JNK signaling pathway. The C. elegans counterpart of this gene is found to regulate synaptic vesicle transport possibly by integrating JNK signaling and kinesin-1 transport. Several alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi membrane; smooth endoplasmic reticulum; dendrite; cytoplasm; axolemma

Molecular Function: JUN kinase binding; MAP-kinase scaffold activity; receptor signaling complex scaffold activity; mitogen-activated protein kinase kinase binding; kinesin binding; mitogen-activated protein kinase kinase kinase binding

Biological Process: vesicle-mediated transport; axon guidance; protein localization; regulation of gene expression; in utero embryonic development; forebrain development; respiratory gaseous exchange; positive regulation of neuron differentiation; alveolus development; regulation of JNK cascade; activation of JNK activity; post-embryonic development