NARF sirna

NARF siRNA (Human)

Cat. Num. AAA8205000

• Gene Names: NARF; IOP2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NARF; NARF siRNA (Human); Nuclear prelamin A recognition factor; Iron-only hydrogenase-like protein 2; IOP2; NARF sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NARF siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 397

• Applicable Applications for NARF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NARF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NARF sirna

siRNA to inhibit NARF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 84452151
• NCBI GeneID: 26502
• NCBI Accession #: NP_001033707.1
• NCBI GenBank Nucleotide #: NM_001038618.2
• UniProt Accession #: Q9UHQ1; Q96AY9; Q9BWC6; A6NCJ3; B3KPX2; K4DI98
• Molecular Weight: 44,681 Da
• NCBI Official Full Name: nuclear prelamin A recognition factor isoform c
• NCBI Official Synonym Full Names: nuclear prelamin A recognition factor
• NCBI Official Symbol: NARF
• NCBI Official Synonym Symbols: IOP2
• NCBI Protein Information: nuclear prelamin A recognition factor
• UniProt Protein Name: Nuclear prelamin A recognition factor
• Protein Family: Nuclear prelamin A recognition factor
• UniProt Gene Name: NARF
• UniProt Synonym Gene Names: IOP2
• UniProt Entry Name: NARF_HUMAN

NCBI Description

Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq, Jul 2008]

Uniprot Description

NARF: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nuclear lamina; nuclear lumen; lamin filament; nucleolus; nucleus

Molecular Function: lamin binding