RLBP1 sirna

RLBP1 siRNA (Human)

Cat. Num. AAA8204537

• Gene Names: RLBP1; CRALBP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RLBP1; RLBP1 siRNA (Human); CRALBP; Retinaldehyde-binding protein 1; Cellular retinaldehyde-binding protein; RLBP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RLBP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 317

• Applicable Applications for RLBP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RLBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RLBP1 sirna

siRNA to inhibit RLBP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4506541
• NCBI GeneID: 6017
• NCBI Accession #: NP_000317.1
• NCBI GenBank Nucleotide #: NM_000326.4
• UniProt Accession #: P12271; B2R667
• Molecular Weight: 36,474 Da
• NCBI Official Full Name: retinaldehyde-binding protein 1
• NCBI Official Synonym Full Names: retinaldehyde binding protein 1
• NCBI Official Symbol: RLBP1
• NCBI Official Synonym Symbols: CRALBP
• NCBI Protein Information: retinaldehyde-binding protein 1
• UniProt Protein Name: Retinaldehyde-binding protein 1
• Protein Family: Retinaldehyde-binding protein
• UniProt Gene Name: RLBP1
• UniProt Synonym Gene Names: CRALBP
• UniProt Entry Name: RLBP1_HUMAN

NCBI Description

The protein encoded by this gene is a 36-kD water-soluble protein which carries 11-cis-retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens. [provided by RefSeq, Jul 2008]

Uniprot Description

RLBP1: Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'. Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD); also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration. Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD). NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss. Defects in RLBP1 are a cause of retinitis punctata albescens (RPA). A rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: cytosol

Molecular Function: transporter activity; 11-cis retinal binding; retinol binding

Biological Process: phototransduction, visible light; visual perception; transport; retinoid metabolic process; vitamin A metabolic process

Disease: Fundus Albipunctatus; Bothnia Retinal Dystrophy; Newfoundland Rod-cone Dystrophy