AHCY sirna

AHCY siRNA (Mouse)

Cat. Num. AAA8204454

• Gene Names: Gm4737; EG11615; AL024110; ENSMUSG00000048087
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AHCY; AHCY siRNA (Mouse); Adenosylhomocysteinase; AdoHcyase; CUBP; Liver copper-binding protein; S-adenosyl-L-homocysteine hydrolase; AHCY sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AHCY siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 432

• Applicable Applications for AHCY sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AHCY gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AHCY sirna

siRNA to inhibit AHCY expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 752272135
• NCBI GeneID: 11615
• NCBI Accession #: NP_001291457.1
• NCBI GenBank Nucleotide #: NM_001304528.1
• UniProt Accession #: P50247; Q91WF1
• Molecular Weight: 47,688 Da
• NCBI Official Full Name: predicted gene 4737
• NCBI Official Synonym Full Names: predicted gene 4737
• NCBI Official Symbol: Gm4737
• NCBI Official Synonym Symbols: EG11615; AL024110; ENSMUSG00000048087
• NCBI Protein Information: predicted gene 4737
• UniProt Protein Name: Adenosylhomocysteinase
• Protein Family: Adenosylhomocysteinase
• UniProt Gene Name: Ahcy
• UniProt Synonym Gene Names: AdoHcyase
• UniProt Entry Name: SAHH_MOUSE

Uniprot Description

SAHH: Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD). A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. Belongs to the adenosylhomocysteinase family.

Protein type: Other Amino Acids Metabolism - selenoamino acid; EC 3.3.1.1; Hydrolase; Amino Acid Metabolism - cysteine and methionine

Cellular Component: neuron projection; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; adenyl nucleotide binding; copper ion binding; protein self-association; adenosylhomocysteinase activity; NAD binding

Biological Process: circadian sleep/wake cycle; S-adenosylmethionine cycle; S-adenosylhomocysteine catabolic process; chronic inflammatory response to antigenic stimulus; response to nutrient