IL33 sirna

IL33 siRNA (Rat)

Cat. Num. AAA8204341

• Gene Names: Il33; RGD1311155
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IL33; IL33 siRNA (Rat); Interleukin-33; IL-33; IL33 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: IL33 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 264

• Applicable Applications for IL33 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat IL33 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IL33 sirna

siRNA to inhibit IL33 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 62079057
• NCBI GeneID: 361749
• NCBI Accession #: NP_001014188.1
• NCBI GenBank Nucleotide #: NM_001014166.1
• UniProt Accession #: Q66H70
• Molecular Weight: 29,486 Da
• NCBI Official Full Name: interleukin-33
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: Il33
• NCBI Official Synonym Symbols: RGD1311155
• NCBI Protein Information: interleukin-33
• UniProt Protein Name: Interleukin-33
• Protein Family: Interleukin
• UniProt Gene Name: Il33
• UniProt Synonym Gene Names: IL-33
• UniProt Entry Name: IL33_RAT

Uniprot Description

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Cellular Component: extracellular space; transport vesicle; chromosome; nucleus

Molecular Function: cytokine activity

Biological Process: positive regulation of cytokine production; negative regulation of T-helper 1 type immune response; T-helper 2 type immune response; positive regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; negative regulation of interferon-gamma production; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; defense response to virus; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response