APOC2 sirna

APOC2 siRNA (Human)

Cat. Num. AAA8204128

• Gene Names: APOC2; APO-CII; APOC-II
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APOC2; APOC2 siRNA (Human); APC2; Apolipoprotein C-II; Apo-CII; ApoC-II; Apolipoprotein C2; APOC2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: APOC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 101

• Applicable Applications for APOC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human APOC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APOC2 sirna

siRNA to inhibit APOC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 32130518
• NCBI GeneID: 344
• NCBI Accession #: NP_000474.2
• NCBI GenBank Nucleotide #: NM_000483.4
• UniProt Accession #: P02655; Q9BS39; Q9UDE3; Q9UNK3; C0JYY4
• Molecular Weight: 11,284 Da
• NCBI Official Full Name: apolipoprotein C-II
• NCBI Official Synonym Full Names: apolipoprotein C-II
• NCBI Official Symbol: APOC2
• NCBI Official Synonym Symbols: APO-CII; APOC-II
• NCBI Protein Information: apolipoprotein C-II
• UniProt Protein Name: Apolipoprotein C-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC2
• UniProt Synonym Gene Names: APC2; Apo-CII; ApoC-II; ProapoC-II
• UniProt Entry Name: APOC2_HUMAN

NCBI Description

This gene encodes a lipid-binding protein belonging to the apolipoprotein gene family. The protein is secreted in plasma where it is a component of very low density lipoprotein. This protein activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells. Mutations in this gene cause hyperlipoproteinemia type IB, characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. This gene is present in a cluster with other related apolipoprotein genes on chromosome 19. Naturally occurring read-through transcription exists between this gene and the neighboring upstream apolipoprotein C-IV (APOC4) gene. [provided by RefSeq, Mar 2011]

Uniprot Description

APOC2: Component of the very low density lipoprotein (VLDL) fraction in plasma, and is an activator of several triacylglycerol lipases. The association of APOC2 with plasma chylomicrons, VLDL, and HDL is reversible, a function of the secretion and catabolism of triglyceride-rich lipoproteins, and changes rapidly. Defects in APOC2 are the cause of hyperlipoproteinemia type 1B (HLPP1B). It is an autosomal recessive trait characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. Belongs to the apolipoprotein C2 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; chylomicron; early endosome; extracellular region

Molecular Function: protein homodimerization activity; phospholipase binding; lipase inhibitor activity; lipid binding; phospholipase activator activity

Biological Process: phototransduction, visible light; positive regulation of fatty acid biosynthetic process; negative regulation of cholesterol transport; lipoprotein metabolic process; cholesterol efflux; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; negative regulation of catalytic activity; positive regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; negative regulation of lipid metabolic process; retinoid metabolic process; lipid catabolic process

Disease: Apolipoprotein C-ii Deficiency