DMAP1 sirna

DMAP1 siRNA (Human)

Cat. Num. AAA8203885

• Gene Names: DMAP1; EAF2; SWC4; MEAF2; DNMAP1; DNMTAP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DMAP1; DMAP1 siRNA (Human); KIAA1425; DNA methyltransferase 1-associated protein 1; DNMAP1; DNMT1-associated protein 1; DMAP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DMAP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 467

• Applicable Applications for DMAP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DMAP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DMAP1 sirna

siRNA to inhibit DMAP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 78000211
• NCBI GeneID: 55929
• NCBI Accession #: NP_001029195.1
• NCBI GenBank Nucleotide #: NM_001034023.1
• UniProt Accession #: Q9NPF5; Q0JSM4; Q5TG41; Q7Z3H7; Q9H0S8; Q9P2C2; A8K001; D3DPY8
• Molecular Weight: 52,993 Da
• NCBI Official Full Name: DNA methyltransferase 1-associated protein 1
• NCBI Official Synonym Full Names: DNA methyltransferase 1 associated protein 1
• NCBI Official Symbol: DMAP1
• NCBI Official Synonym Symbols: EAF2; SWC4; MEAF2; DNMAP1; DNMTAP1
• NCBI Protein Information: DNA methyltransferase 1-associated protein 1
• UniProt Protein Name: DNA methyltransferase 1-associated protein 1
• Protein Family: DNA methyltransferase 1-associated protein
• UniProt Gene Name: DMAP1
• UniProt Synonym Gene Names: KIAA1425; DNMAP1; DNMT1-associated protein 1
• UniProt Entry Name: DMAP1_HUMAN

NCBI Description

This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

DMAP1: Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX- mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: nucleoplasm; NuA4 histone acetyltransferase complex; cytoplasm; replication fork; nucleus

Molecular Function: protein binding; chromatin binding; transcription corepressor activity

Biological Process: chromatin remodeling; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of growth; positive regulation of transcription factor import into nucleus; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; DNA repair; negative regulation of transcription, DNA-dependent