LPGAT1 sirna

LPGAT1 siRNA (Mouse)

Cat. Num. AAA8203608

• Gene Names: Lpgat1; AI649174; AW112037; BC013667
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LPGAT1; LPGAT1 siRNA (Mouse); FAM34A; Acyl-CoA:lysophosphatidylglycerol acyltransferase 1; LPGAT1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LPGAT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 370

• Applicable Applications for LPGAT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LPGAT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LPGAT1 sirna

siRNA to inhibit LPGAT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 26986567
• NCBI GeneID: 226856
• NCBI Accession #: NP_758470.1
• NCBI GenBank Nucleotide #: NM_172266.3
• UniProt Accession #: Q91YX5
• Molecular Weight: 43,088 Da
• NCBI Official Full Name: acyl-CoA:lysophosphatidylglycerol acyltransferase 1 isoform 2
• NCBI Official Synonym Full Names: lysophosphatidylglycerol acyltransferase 1
• NCBI Official Symbol: Lpgat1
• NCBI Official Synonym Symbols: AI649174; AW112037; BC013667
• NCBI Protein Information: acyl-CoA:lysophosphatidylglycerol acyltransferase 1
• UniProt Protein Name: Acyl-CoA:lysophosphatidylglycerol acyltransferase 1
• Protein Family: Acyl-CoA:lysophosphatidylglycerol acyltransferase
• UniProt Gene Name: Lpgat1
• UniProt Synonym Gene Names: Fam34a
• UniProt Entry Name: LGAT1_MOUSE

Uniprot Description

LPGAT1: Lysophoshatidylglycerol (LPG) specific acyltransferase that recognizes various acyl-CoAs and LPGs as substrates but demonstrates a clear preference for long chain saturated fatty acyl-CoAs and oleoyl-CoA as acyl donors. Prefers oleoyl-LPG over palmitoyl-LPG as an acyl receptor and oleoyl-CoA over lauroyl-CoA as an acyl donor. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: EC 2.3.1.-; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Cellular Component: membrane; endoplasmic reticulum; cytoplasm; integral to membrane

Molecular Function: keto acid formate lyase activity; glucosaminyl-phosphotidylinositol O-acyltransferase activity; S-malonyltransferase activity; 3-hydroxybutyryl-CoA thiolase activity; S-acetyltransferase activity; O-octanoyltransferase activity; O-sinapoyltransferase activity; acyl-CoA N-acyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; succinyltransferase activity; Ras palmitoyltransferase activity; palmitoyltransferase activity; C-acyltransferase activity; C-palmitoyltransferase activity; dihydrolipoamide S-acyltransferase activity; protein-cysteine S-acyltransferase activity; N-acetyltransferase activity; serine O-acyltransferase activity; O-succinyltransferase activity; octanoyltransferase activity; malonyltransferase activity; O-palmitoyltransferase activity; sterol O-acyltransferase activity; 3-ketopimelyl-CoA thiolase activity; carnitine O-acyltransferase activity; S-succinyltransferase activity; N-acyltransferase activity; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase activity; N-palmitoyltransferase activity; O-acyltransferase activity; acylglycerol O-acyltransferase activity; O-acetyltransferase activity; benzoyl acetate-CoA thiolase activity; transferase activity; sinapoyltransferase activity; protein-cysteine S-myristoyltransferase activity; S-acyltransferase activity; acetyltransferase activity; myristoyltransferase activity; N-succinyltransferase activity; transferase activity, transferring acyl groups; L-2-aminoadipate N-acetyltransferase activity

Biological Process: metabolic process; lipid metabolic process; phospholipid biosynthetic process