BCAP31 sirna

BCAP31 siRNA (Mouse)

Cat. Num. AAA8203602

• Gene Names: Bcap31; Bap31
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BCAP31; BCAP31 siRNA (Mouse); BAP31; B-cell receptor-associated protein 31; BCR-associated protein 31; Bap31; p28; BCAP31 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BCAP31 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 245

• Applicable Applications for BCAP31 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BCAP31 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BCAP31 sirna

siRNA to inhibit BCAP31 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31981310
• NCBI GeneID: 27061
• NCBI Accession #: NP_036190.2
• NCBI GenBank Nucleotide #: NM_012060.4
• UniProt Accession #: Q61335; Q9D0E9; Q9D8G7; A2ALM8
• Molecular Weight: 27,957 Da
• NCBI Official Full Name: B-cell receptor-associated protein 31
• NCBI Official Synonym Full Names: B cell receptor associated protein 31
• NCBI Official Symbol: Bcap31
• NCBI Official Synonym Symbols: Bap31
• NCBI Protein Information: B-cell receptor-associated protein 31
• UniProt Protein Name: B-cell receptor-associated protein 31
• Protein Family: B-cell receptor-associated protein
• UniProt Gene Name: Bcap31
• UniProt Synonym Gene Names: Bap31; BCR-associated protein 31; Bap31
• UniProt Entry Name: BAP31_MOUSE

Uniprot Description

BCAP31: May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. Homodimer and heterodimer with BCAP29. Binds CASP8 (isoform 9) as a complex containing BCAP31, BCAP29, BCL2 and/or BCL2L1. Interacts with VAMP3, VAMP1 and membrane IgD immunoglobulins. May interact with ACTG1 and non-muscle myosin II. Interacts with PTPLB. Ubiquitous. Belongs to the BCAP29/BCAP31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: endoplasmic reticulum membrane; mitochondrion; clathrin-coated vesicle; endoplasmic reticulum; cell; integral to plasma membrane; integral to membrane; lipid particle; cytosol; Golgi membrane; membrane; cytoplasm; plasma membrane; intracellular

Molecular Function: protein binding; MHC class I protein binding; protein complex binding

Biological Process: vesicle-mediated transport; intracellular protein transport; ER to Golgi vesicle-mediated transport; protein transport; elevation of cytosolic calcium ion concentration; apoptosis; transport; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of caspase activity; immune response; elevation of mitochondrial calcium ion concentration