CTSK sirna

CTSK siRNA (Human)

Cat. Num. AAA8203210

• Gene Names: CTSK; CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTSK; CTSK siRNA (Human); CTSO; CTSO2; Cathepsin K; Cathepsin O; Cathepsin O2; Cathepsin X; CTSK sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CTSK siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 329

• Applicable Applications for CTSK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CTSK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTSK sirna

siRNA to inhibit CTSK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4503151
• NCBI GeneID: 1513
• NCBI Accession #: NP_000387.1
• NCBI GenBank Nucleotide #: NM_000396.3
• UniProt Accession #: P43235; Q6FHS6
• Molecular Weight: 36,966 Da
• NCBI Official Full Name: cathepsin K preproprotein
• NCBI Official Synonym Full Names: cathepsin K
• NCBI Official Symbol: CTSK
• NCBI Official Synonym Symbols: CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
• NCBI Protein Information: cathepsin K
• UniProt Protein Name: Cathepsin K
• Protein Family: Cathepsin
• UniProt Gene Name: CTSK
• UniProt Synonym Gene Names: CTSO; CTSO2
• UniProt Entry Name: CATK_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]

Uniprot Description

CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family.

Protein type: Protease; EC 3.4.22.38

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; extracellular region

Molecular Function: collagen binding; protein binding; proteoglycan binding; fibronectin binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: collagen catabolic process; extracellular matrix disassembly; intramembranous ossification; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; proteolysis; bone resorption

Disease: Pycnodysostosis