CHRNA3 sirna

CHRNA3 siRNA (Mouse)

Cat. Num. AAA8202853

• Gene Names: Chrna3; (a)3; Acra3; Acra-3; A730007P14Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHRNA3; CHRNA3 siRNA (Mouse); Neuronal acetylcholine receptor subunit alpha-3; CHRNA3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHRNA3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 504

• Applicable Applications for CHRNA3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHRNA3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHRNA3 sirna

siRNA to inhibit CHRNA3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 70778736
• NCBI GeneID: 110834
• NCBI Accession #: NP_660111.2
• NCBI GenBank Nucleotide #: NM_145129.2
• UniProt Accession #: Q8R4G9; Q8BV44
• Molecular Weight: 57,110 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-3
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha polypeptide 3
• NCBI Official Symbol: Chrna3
• NCBI Official Synonym Symbols: (a)3; Acra3; Acra-3; A730007P14Rik
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-3
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-3
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: Chrna3
• UniProt Entry Name: ACHA3_MOUSE

Uniprot Description

nAChRA3: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 3/CHRNA3 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, cation; Channel, ligand-gated

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; membrane; cell soma; postsynaptic density; dendrite; plasma membrane; integral to membrane; synapse; cell junction

Molecular Function: protein binding; protein heterodimerization activity; acetylcholine receptor activity; ion channel activity; drug binding; acetylcholine binding; nicotinic acetylcholine-activated cation-selective channel activity; extracellular ligand-gated ion channel activity

Biological Process: regulation of smooth muscle contraction; response to drug; nervous system development; transmembrane receptor protein tyrosine kinase activation (dimerization); protein heterooligomerization; regulation of muscle contraction; regulation of dendrite morphogenesis; regulation of acetylcholine secretion; behavioral response to nicotine; locomotory behavior; signal transduction; synaptic transmission, cholinergic; synaptic transmission; regulation of membrane potential; transport; ion transport; synaptic transmission involved in micturition; regulation of excitatory postsynaptic membrane potential; cation transport