AGFG1 sirna

AGFG1 siRNA (Human)

Cat. Num. AAA8202755

• Gene Names: AGFG1; HRB; RAB; RIP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AGFG1; AGFG1 siRNA (Human); HRB; RAB; RIP; Arf-GAP domain and FG repeat-containing protein 1; HIV-1 Rev-binding protein; Nucleoporin-like protein RIP; Rev-interacting protein; Rev/Rex activation domain-binding protein; AGFG1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AGFG1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 584

• Applicable Applications for AGFG1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AGFG1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AGFG1 sirna

siRNA to inhibit AGFG1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 206597505
• NCBI GeneID: 3267
• NCBI Accession #: NP_001128659.1
• NCBI GenBank Nucleotide #: NM_001135187.1
• UniProt Accession #: P52594; Q15277; Q4VAS0; Q4VAS1; Q4VAS3; Q53QT8; Q53R11; B3KUL1; E9PHX7
• Molecular Weight: 60,767 Da
• NCBI Official Full Name: arf-GAP domain and FG repeat-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: ArfGAP with FG repeats 1
• NCBI Official Symbol: AGFG1
• NCBI Official Synonym Symbols: HRB; RAB; RIP
• NCBI Protein Information: arf-GAP domain and FG repeat-containing protein 1
• UniProt Protein Name: Arf-GAP domain and FG repeat-containing protein 1
• Protein Family: Arf-GAP domain and FG repeat-containing protein
• UniProt Gene Name: AGFG1
• UniProt Synonym Gene Names: HRB; RAB; RIP
• UniProt Entry Name: AGFG1_HUMAN

NCBI Description

The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

RIP: Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element- containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. Interacts with EPS15R and EPS15. Interacts with FCHO1. Ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nuclear export

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: cell projection; cell soma; intracellular membrane-bound organelle; cytoplasmic membrane-bound vesicle; nuclear pore

Molecular Function: protein binding; DNA binding; zinc ion binding; RNA binding

Biological Process: acrosome formation; mRNA export from nucleus; multicellular organismal development; spermatid nuclear differentiation; intermediate filament organization; positive regulation of GTPase activity